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Transcriptome profile of wildtype and SEDL knockdown Medaka embryos and Human ARPE-19 cells

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens Oryzias latipes

PROVIDER: GSE143540 | GEO | 2024/05/03

REPOSITORIES: GEO

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Transcriptome profile of wildtype and SEDL knockdown Medaka embryos and Human ARPE-19 cells

Project description:Transcriptome profile of wildtype and SEDL knockdown Medaka embryos and Human ARPE-19 cells

| PRJNA600924 | ENA

The function of tcf3 in medaka embryos: efficient knockdown with pePNAs.

Project description:BACKGROUND:The application of antisense molecules, such as morpholino oligonucleotides, is an efficient method of gene inactivation in vivo. We recently introduced phosphonic ester modified peptide nucleic acids (PNA) for in vivo loss-of-function experiments in medaka embryos. Here we tested novel modifications of the PNA backbone to knockdown the medaka tcf3 gene. RESULTS:A single tcf3 gene exists in the medaka genome and its inactivation strongly affected eye development of the embryos, leading to size reduction and anophthalmia in severe cases. The function of Tcf3 strongly depends on co-repressor interactions. We found interactions with Groucho/Tle proteins to be most important for eye development. Using a dominant negative approach for combined inactivation of all groucho/tle genes also resulted in eye phenotypes, as did interference with three individual tle genes. CONCLUSIONS:Our results show that side chain modified PNAs come close to the knockdown efficiency of morpholino oligonucleotides in vivo. A single medaka tcf3 gene combines the function of the two zebrafish paralogs hdl and tcf3b. In combination with Groucho/Tle corepressor proteins Tcf3 acts in anterior development and is critical for eye formation.

| S-EPMC5759164 | biostudies-literature

Transcriptome profile of Stage 40 Oryzias latipes (Medaka) embryos

Project description:Transcriptomic profile of Stage 40 Oryzias latipes (Medaka) embryos wild type and KO for SEDL

2024-05-03 | GSE143538 | GEO

Transcriptome profile of Stage 40 Oryzias latipes (Medaka) embryos II

Project description:In order to identify the effects of the absence of SEDL on Medaka transcriptome, we performed RNAseq experiments Transcriptome analysis of the liver of mice trated with CD95 activating antibody, compared with liver of mice trated with saline.

2024-05-03 | GSE186769 | GEO

Transcriptome profile of Stage 40 Oryzias latipes (Medaka) embryos

Project description:Transcriptome profile of Stage 40 Oryzias latipes (Medaka) embryos

| PRJNA600933 | ENA

Chromatin organization in NPP-13 knockdown C. elegans embryos

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-08-15 | E-GEOD-50488 | biostudies-arrayexpress

Transcriptome sequencing of ARPE-19 cells treated with cinobufagin

Project description:This study investigates the alterations in the transcriptomic landscape of cells following treatment with cinobufagin.

2024-09-10 | GSE276415 | GEO

Transcriptome profile of Stage 40 Oryzias latipes (Medaka) embryos II

Project description:Transcriptome profile of Stage 40 Oryzias latipes (Medaka) embryos II

| PRJNA775990 | ENA

Bisulfite sequencing of medaka

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-11-03 | GSE160114 | GEO

Chromatin organization in NPP-13 knockdown C. elegans embryos

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-08-15 | GSE50488 | GEO

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