Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Acquisition of Cancer Stem Cell Properties in Osteosarcoma Cells by Defined Factors

ABSTRACT: Cancer stem cells (CSCs) are proposed to be unique subsets of clones within a tumor, attributed with tumor progression, resistance to chemotherapy, and metastases. However, little is known about the molecular mechanisms regulating the acquisition and maintenance of CSC properties in cancers, especially in rare cancers, such as osteosarcoma (OS). In this study, we generated OS cells with CSC properties following transduction of defined factors. This method enabled us to obtain abundant OS CSC material. The results of our study could facilitate a better understanding of the mechanisms of OS progression, and aid in the development of new therapies targeting CSCs in patients with OS.

ORGANISM(S): Homo sapiens

PROVIDER: GSE143556 | GEO | 2020/01/14

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Expression profile of osteosarcoma cells in which Sox2 maintains cancer stem cells

Project description:Sox2 is required to maintain osteosarcoma cell tumor initiation.Knockdown of Sox2 leads tpo loss of tumorigenic properties. To examine gene expression changes upon Sox2 knockdown, we performed microarray analysis on mouse osteosarcoma cells expressing scrambled or Sox2shRNA. We found that genes upregulated upon Sox2 knockdown included osteoblast diffrentiation genes and genes down regulated included cell cycle and RNA processing genes as well as YAP-TEAD target genes. The Hippo pathway has a profound tumor suppressive role in cancer by restraining the strong growth-promoting function of YAP. We have previously shown that the stem cell transcription factor Sox2 maintains the tumorigenicity of osteosarcoma cancer stem cells (CSCs). In this report, we describe that Sox2 maintains stemness by antagonizing the Hippo pathway via direct repression of Hippo activators, Nf2 (Merlin) and WWC1 (Kibra), thereby leading to exaggerated YAP function. YAP is potently oncogenic in osteosarcoma and its depletion sharply reduces the tumorigenic CSC fraction. Low Nf2, low WWC1, and high YAP expression mark the CSC fraction of the tumor population, while the more differentiated fraction has high Nf2, high WWC1 and reduced YAP expression. This Sox2-Hippo axis is conserved and also operates in other Sox2-dependent cancers such as glioblastomas. We propose that disruption of YAP transcriptional activity reduces CSCs and could be a therapeutic strategy for Sox2- dependent tumors. Gene expression of 482 mouse lines mOS482 were analyzed by microarray. 3 replicates each of scrambled and Sox2shRNA were processed and hybridized

2015-02-15 | E-GEOD-64965 | biostudies-arrayexpress

Expression profile of osteosarcoma cells in which Sox2 maintains cancer stem cells

2015-02-15 | GSE64965 | GEO

Microarray profiling analysis of whole transcriptome expression in osteosarcoma

Project description:Osteosarcoma (OS)is a rare primary malignant bone tumor in adolescents and children with a poor prognosis. Identification of prognostic genes lags far behind the advance of the treatments. We identified differential genes by microarray analysis from paired OS tissues. Hub genes, gene set enrichment analysis, and pathway analysis were performed to gain an insight into the pathway alterations of OS. These results showed CPE could be served as a prognostic factor in osteoblastic OS and should be further investigated as potential therapeutic target. The present study evaluated the whole transcriptome expression of osteosarcoma progression and provided novel therapeutic targets for advanced osteosarcoma.

2023-03-01 | GSE225588 | GEO

the role of hsa_circ_0000073 in Osteosarcoma

Project description:Osteosarcoma (OS) is one of the leading causes of cancer mortality in children and teenagers. Patients with OS have been shown to have a poor prognosis and may suffer from potential distant metastasis. Emerging evidence has revealed that circular RNAs (circRNAs) are involved in OS progression. In this paper, we aim to explore the function of hsa_circ_0000073 in the progression of OS. OS cells transfected with sh‐NC or sh-circ_0000073 were enrolled in this study.We used microarray analysis to identify the mRNA expression in the cells.

2021-08-24 | GSE176373 | GEO

Acquisition of Cancer Stem Cell Properties in Osteosarcoma Cells by Defined Factors

Project description:Acquisition of Cancer Stem Cell Properties in Osteosarcoma Cells by Defined Factors

| PRJNA600943 | ENA

Dataset #2 for genome-wide analysis of heritable dog osteosarcoma in 3 breeds

2013-11-12 | GSE51976 | GEO

Dataset #1 for genome-wide analysis of heritable dog osteosarcoma in 3 breeds

2013-11-12 | GSE51973 | GEO

Dataset #3 for genome-wide analysis of heritable dog osteosarcoma in 3 breeds

2013-11-12 | GSE51734 | GEO

SPRR1A is a potential therapeutic target for osteosarcoma: in vitro and in vivo evaluations using novel generated artificial osteosarcoma cancer stem cell-like cells

Project description:Osteosarcoma (OS) is the most prevalent primary malignant bone tumor.We have recently succeeded in generating CSC-like cells (MG-OKS) from the OS cell line MG-63 by transducing defined factors. A significant increase was observed in small proline-rich protein 1A (SPRR1A) expression, a cross-linked envelope protein in keratinocytes, in MG-OKS cells. This study aimed to evaluate the role of SPRR1A in OS using novel MG-OKS cells. MG-OKS cells were transfected with SPRR1A siRNA (siMG-OKS) to suppress SPRR1A expression selectively; MG-OKS and scrambled siRNA-transfected MG-OKS (scMG-OKS) cells served as controls. Our date that genes involved in cell adhesion were suppressed in the siMG-OKS group, suggesting that suppression of cell adhesion may cause phenotypic changes.

2024-11-01 | GSE268670 | GEO

Dataset #3 for genome-wide analysis of heritable dog osteosarcoma in 3 breeds

Project description:Osteosarcoma in dogs is a spontaneously occurring disease with a global tumor gene expression signature indistinguishable from human pediatric tumors and clinical progression is remarkably similar. Unlike human OS, canine OS is a highly heritable disease with some large and giant dog breeds at >10x increased risk. We did a genome wide association study of osteosarcoma using the Illumina CanineHD genotyping array in three breeds: greyhound (mortality from OS = 26%), rottweiler (17%) and Irish wolfhound (IWH, 21%) and identified 33 inherited risk loci explaining 55 to 85% of phenotype variance in each breed. Data created for a genome-wide association studio of heritable osteosarcoma risk factors in dogs, including 267 racing greyhounds (153 affected (A) + 114 unaffected (U)), 135 rottweilers (80 A + 55 U), 141 IWH (76 A + 65 U) and 19 AKC greyhounds using the Illumina CanineHD array (~170,000 SNPs genomewide)

2013-11-12 | E-GEOD-51734 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data