Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Deregulation of ribosomal protein expression and translation promotes breast cancer metastasis

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE143626 | GEO | 2020/02/07

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Deregulation of ribosomal protein expression and translation promotes breast cancer metastasis.

Project description:Circulating tumor cells (CTCs) are shed into the bloodstream from primary tumors, but only a small subset of these cells generates metastases. We conducted an in vivo genome-wide CRISPR activation screen in CTCs from breast cancer patients to identify genes that promote distant metastasis in mice. Genes coding for ribosomal proteins and regulators of translation were enriched in this screen. Overexpression of RPL15, which encodes a component of the large ribosomal subunit, increased metastatic growth in multiple organs and selectively enhanced translation of other ribosomal proteins and cell cycle regulators. RNA sequencing of freshly isolated CTCs from breast cancer patients revealed a subset with strong ribosome and protein synthesis signatures; these CTCs expressed proliferation and epithelial markers and correlated with poor clinical outcome. Therapies targeting this aggressive subset of CTCs may merit exploration as potential suppressors of metastatic progression.

| S-EPMC7307008 | biostudies-literature

Deregulation of ribosomal protein expression and translation promotes breast cancer metastasis [rna-Seq BRx142]

Project description:We conducted an in vivo genome-wide CRISPR activation screen to identify genes that accelerate distal metastasis by breast cancer patient-derived circulating tumor cells (CTCs) following direct intravascular inoculation in mice. Regulators of translation and ribosomal proteins were prominent among these, and expression of RPL15, a component of the large ribosome subunit, was sufficient to increase metastatic growth in multiple organs. RPL15 overexpression selectively increases translation of other ribosomal proteins and cell cycle regulators. Unsupervised analysis of single-cell RNA sequencing of freshly-isolated CTCs from breast cancer patients identifies a subset with strong ribosomal and protein translation signatures, correlated with increased proliferative markers, epithelial markers and poor clinical outcome. Thus, ribosome protein expression identifies an aggressive subset of CTCs, whose therapeutic targeting may suppress metastatic progression.

2020-02-07 | GSE143624 | GEO

Deregulation of ribosomal protein expression and translation promotes breast cancer metastasis [ribo-Seq BRx142]

2020-02-07 | GSE143623 | GEO

Deregulation of ribosomal protein expression and translation promotes breast cancer metastasis [rna-uArray MCF10A]

2020-02-07 | GSE143625 | GEO

Deregulation of ribosomal protein expression and translation promotes breast cancer metastasis [rna-Seq CTCs 1]

2020-02-07 | GSE144494 | GEO

Deregulation of ribosomal protein expression and translation promotes breast cancer metastasis [rna-Seq CTCs 2]

2020-02-07 | GSE144495 | GEO