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Expression data from adipocytes and white adipose tissue of mice maintained on normal chow or high fat diet

ABSTRACT: We studied the role of the cAMP responsive factor CREB in promoting insulin resistance following its activation in adipose under obese conditions We identified genes that were upregulated in primary cultures of mouse adipocytes following exposure to forskolin; and we characterized genes that are also induced in white adipose tissue in mice maintained on a high fat diet. Keywords: signaling study

ORGANISM(S): Mus musculus

PROVIDER: GSE14363 | GEO | 2009/03/03

SECONDARY ACCESSION(S): PRJNA111399

REPOSITORIES: GEO

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Transcription profiling of mouse adipocytes and white adipose tissue of mice maintained on normal chow or high fat diet

Project description:We studied the role of the cAMP responsive factor CREB in promoting insulin resistance following its activation in adipose under obese conditions; We identified genes that were upregulated in primary cultures of mouse adipocytes following exposure to forskolin; and we characterized genes that are also induced in white adipose tissue in mice maintained on a high fat diet. Experiment Overall Design: Primary cultures of mouse adipocytes,harvested from visceral adipose tissue, were exposed to forskolin (10uM) or vehicle control (DMSO) for 2 hours. White adipose tissue (epididymal fat pads) were collected from mice maintained on a 60% high fat diet for 12 weeks and from control mice on normal chow.

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