Transcriptomics

Dataset Information

0

Aryl hydrocarbon receptor mediates larval zebrafish fin duplication following exposure to benzofluoranthenes.


ABSTRACT: Polycyclic aromatic hydrocarbons (PAHs) can alter gene expression by acting through the aryl hydrocarbon receptor (AHR). In a previous phenotypic screen of over 120 PAHs, we identified four PAHs that induce an ectopic caudal fin (called X-fin) in larval zebrafish: benzo[k]fluoranthene (BkF), dibenzo[b,k]fluoranthene, dibenzo[a,h]anthracene, and benzo[j]fluoranthene. We investigated several plausible mechanisms of X-fin formation using the most potent X-fin inducer, BkF. The X-fin phenotype was dependent on the AHR paralog Ahr2, and we performed RNA sequencing to identify altered gene expression patterns. Transcriptional profiles of distal trunk tissue, where the phenotype was manifest, were generated for animals exposed to 1% DMSO (control) or 12 μM BkF. Four time points of X-fin development were considered for transcriptomics: prior to visual emergence of X-fin (48 hpf), during manifestation of disrupted caudal fin fold development (60 and 72 hpf), and after emergence of X-fin (96 hpf).

ORGANISM(S): Danio rerio

PROVIDER: GSE143945 | GEO | 2020/01/21

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2012-04-17 | E-GEOD-37326 | biostudies-arrayexpress
2017-01-01 | GSE87689 | GEO
2015-10-01 | GSE51315 | GEO
2017-01-01 | GSE87688 | GEO
2015-10-01 | GSE51317 | GEO
2013-07-22 | E-GEOD-49068 | biostudies-arrayexpress
2020-03-18 | GSE128492 | GEO
2020-03-18 | GSE128471 | GEO
2013-05-15 | E-GEOD-44130 | biostudies-arrayexpress
2008-04-07 | E-GEOD-10184 | biostudies-arrayexpress