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Transcriptomic microRNA profiling of dendritic cells in response to gut microbiota secreted vesicles

ABSTRACT: Extracellular membrane vesicles (MVs) released by gut microbiota are key players in the communication with the host. Next-generation sequencing of small RNAs was used to quantify miRNA expression in monocyte-derived dendritic cells after 24 h-stimulation with MVs isolated from two E. coli intestinal isolates, the probiotic E. coli strain Nissle 1917 (EcN) and the commensal ECOR12. Analysis revealed miRNAs differentially expressed in response to MVs compared to immature control dendritic cells (log2fold-change > 0.7 and padj < 0.001). A common set of miRNAs was modulated by MVs from both strains (46 downregulated, 75 upregulated). In addition, these vesicles elicited differential expression of specific miRNAs depending on the producer strain (26 downregulated and 23 upregulated by EcN; 48 downregulated and 49 upregulated by ECOR12).

ORGANISM(S): Homo sapiens

PROVIDER: GSE144355 | GEO | 2020/06/25

REPOSITORIES: GEO

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E. coli Nissle 1917 transcriptional response after treatment with Calprotectin

Project description:To determine whether calprotectin can elicit any transcriptional response in the probiotic E. coli Nissle 1917(EcN), EcN was treated with 200 ug/g of calprotectin in log phase.

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The probiotic Escherichia coli strain Nissle 1917 combats the lambdoid bacteriophages stx and λ

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Analysis of the Escherichia coli membrane vesicle proteome identifies markers of purity and culture conditions

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