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Hi-CoP, a simple and robust assay for detecting interactions of regulatory regions

ABSTRACT: First we developed a new, simple, and robust assay called CoP (Column Purified chromatin) for profiling of accessible regions by directly purifying fragmentized crosslinked chromatin with DNA purification column. The CoP chromatin regions by CoP-seq are consistent with the accessible regions detected by ATAC-seq and FARIE-seq. Then we integrated CoP-seq and Hi-C technique (Hi-CoP) for interactions of accessible chromatin regions which represent active cis-regulatory elements in cells. Our data showed that Hi-CoP assay can robustly detect interactions of regulatory regions.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE144412 | GEO | 2020/01/29

REPOSITORIES: GEO

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