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PDGFRβ+ cells play a dual role as hematopoietic precursors and niche cells during mouse ontogeny

ABSTRACT: Hematopoietic stem cell (HSC) generation in the aorta-gonads-mesonephros region requires HSC specification signals from the surrounding microenvironment. In zebrafish, PDGF-B/PDGFRβ signaling controls hematopoietic stem/progenitor cell (HSPC) generation and is required in the HSC specification niche. Little is known about murine HSPC specification in vivo and whether PDGF-B/PDGFRβ is involved. Here we show that PDGFRβ is expressed in distinct perivascular stromal cell layers surrounding the mid-gestation dorsal aorta, and its deletion impairs hematopoiesis. We demonstrate that PDGFRβ+ cells play a dual role in murine hematopoiesis. They act in the aortic niche to support HSPCs, and in addition, PDGFRβ+ embryonic precursors give rise to a subset of HSPCs that persist into adulthood. These findings provide crucial information for the controlled production of these clinically important cells in vitro.

ORGANISM(S): Mus musculus

PROVIDER: GSE144837 | GEO | 2023/02/05

REPOSITORIES: GEO

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PDGFRβ+ cells play a dual role as hematopoietic precursors and niche cells during mouse ontogeny

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