ABSTRACT: We performed RNAseq to identify miRNAs that were specifically down regulated in neutrophils, and RNAseq for identification of mRNA targets of miRNA-99 in neutrophils
Project description:We performed RNAseq to identify miRNAs that were specifically down regulated in neutrophils, and RNAseq for identification of mRNA targets of miRNA-31, miRNA-190 and miRNA-375 in neutrophils
Project description:We performed RNAseq to identify miRNAs that were specifically down regulated in neutrophils, and RNAseq for identification of mRNA targets of miRNA-199 in neutrophils
Project description:We FACS-purified GFP+ LSK cells from mice transplanted with stably engrafted miR-99 KD and Scr HSCs and performed RNA-sequencing, demonstrating miR-99 KD results in significant depletion of hematopoietic stem cell gene expression signature and induces a differentiated progenitor gene expression signature.
Project description:Human neutrophils were isolated with negative selection (Miltenyi MacsXpress, purity > 99%) from fresh venous blood. RNA was isolated directly after without stimulating the cells.
Project description:RNA expression in Srf wildtype and knockout primary bone marrow derived mature neutrophils was determined via RNA sequencing Mature Gr1 high, 7/4 high, SSC high neutrophils were sorted from Srf WT and KO mouse bone marrow and submitted for RNA sequencing