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The expression of mRNAs after H19 overexpression in periodontal ligament cells

ABSTRACT: RNA (4 μg) extracted from PDLCs was treated with DNase. After removing the ribosomal RNA, the RNA library was prepared. RNA sequencing was performed on a HiSeq 2000 System (Illumina, San Diego, CA, USA). After the raw data were trimmed and filtered, the remaining data were then mapped to the human genome (hg19) using HISAT2. Differential gene expression was evaluated using the EBSeq package of the Bioconductor R program as previously described. To preliminarily study the mechanism of H19 in the regulation of autophagy, RNA sequencing after transfection with the H19 vector was performed. The RNA sequencing data showed that 1120 mRNAs, including 431 upregulated and 689 downregulated mRNAs, were differentially expressed in the H19 overexpression group compared to the control group. The differential expression levels of mRNAs were visually expressed with a volcano plot. To evaluate the function of differentially expressed genes, KEGG pathway analyses were performed. The data showed that the differentially expressed mRNAs were enriched in the PI3K/AKT signaling pathway, which is relevant to autophagy regulation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE145112 | GEO | 2020/02/12

REPOSITORIES: GEO

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The expression of mRNAs after H19 overexpression in periodontal ligament cells

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