Project description:RNA was isolated from 23 old barley plants (shoots), line Rolap. Libraries were prepared using mRNA-Seq Library kit v2 (Lexogen), followed by sequencing of NGS libraries
Project description:RNA was isolated from 23 old barley plants (shoots and roots), line Rolap. We used a modified method that allows for enrichment of small RNAs. Libraries were prepared using TruSeq Small RNA Library Preparation Kit (Illumina), followed by sequencing of NGS libraries.
Project description:RNA was isolated from 23 old barley plants (shoots and roots), line Rolap. PARE libraries were constructed for both barley organs, followed by sequencing of NGS libraries.