Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome profiling following knockdown of NAA50 in Arabidopsis

ABSTRACT: We utilized RNA-seq to identify transcriptome changes following knockdown of the Arabidopsis N-terminal acetyltransferase NAA50. Transgenic Arabidopsis plants carrying a dexamethasone-inducible amiRNA were used to study the effects of NAA50 knockdown. Significant changes occurred in the transcriptomes of NAA50 knockdown plants at 12 and 24 hours after dexamethasone treatment. These changes demonstrate that loss of NAA50 results in a reduction in signaling related to plant growth and development, as well as an increase in the expression of stress- and defense-related pathways.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE145580 | GEO | 2020/02/20

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcription profiling of Arabidopsis VCS-amiRNA plants under a dehydration stress condition

Project description:Transcriptional profiles were compared between wild type and the VCS-amiRNA plants under a dehydration stress condition. VCS-amiRNA plants are VCS (VARICOSE)-knockdown Arabidopsis plants expressing an artificial miRNA (ami-RNA) construct for VCS. VCS-amiRNA plants showed a reduction in VCS gene expression to approximately 40% of that of wild type plants.

2018-01-31 | E-MTAB-5004 | biostudies-arrayexpress

Arabidopsis proteome in the tripartite interaction between cytokinin, light and temperature

Project description:Transgenic Arabidopsis plants carrying dexamethasone-inducible barley cytokinin oxidase/dehydrogenase (CaMV35S>GR>HvCKX2) and agrobacterial isopentenyl transferase (CaMV35S>GR>ipt) constructs were profiled to elucidate proteome-wide response to cold stress under different light conditions.

2021-01-04 | PXD020480 | Pride

Transcription profiling under heat stress of transgenic Arabidopsis lines in which expression of BPMs were suppressed by an amiRNA

Project description:BPMs are a family of E3 ubiquitin ligases. We identified BPM proteins as interactors of the Arabidopsis stress responsive transcription factor DREB2A. We found an increase in the accumulation level of the DREB2A protein under heat stress in transgenic Arabidopsis plants in which expression of six BPMs were suppressed by an amiRNA. In order to evaluate the impacts of the amiRNA on the gene expression under heat stress, transcriptomes were compared between these transgenic plants and vector control plants.

2018-11-01 | E-MTAB-5845 | biostudies-arrayexpress

Transcription profiling of Hsp70-14 and -15 silenced Arabidopsis plants

Project description:AtHsp70-14 and -15 are two closely related isoforms of the Hsp110/SSE subfamily of the HSP70 chaperones in Arabidopsis thaliana. By analysing T-DNA insertion and amiRNA-AtHsp70-14/15 lines, we could demonstrate an essential function of AtHsp70-15 for plant growth. AtHsp70-15 silenced plants are reduced in size and stomata opening is impaired which results in accelerated wilting of detached leaves. AtHsp70-15 silenced plants are also more tolerant to TuMV infection but more sensitive to heat treatment. Therefore comparative transcriptome analysis of amiRNA-AtHsp70-14/15 and control plants, PME1, was performed with leaf samples of 8 week old plants grown under ambient conditions. The analysis of differentially regulated genes compared to control plants exhibited a constitutive heat stress response / cytosolic protein response.

2011-04-29 | E-MEXP-2866 | biostudies-arrayexpress

Transcription profiling of AtHsp70-14 and AtHsp70-15 deficient Arabidopsis thaliana plants

Project description:AtHsp70-14 and -15 (At1g79930 and At1g79920) are two closely related isoforms of the Hsp110/SSE subfamily of the HSP70 chaperones in Arabidopsis thaliana. By analysing T-DNA insertion and amiRNA-AtHsp70-14/15 lines, we could demonstrate an essential function of AtHsp70-15 for plant growth. AtHsp70-15 deficient plants are reduced in size and stomata opening is impaired which results in accelerated wilting of detached leaves. Hsp70-15 deficient plants are also more tolerant to TuMV infection but more sensitive to heat treatment. Therefore comparative transcriptome analysis of amiRNA-AtHsp70-14/15, hsp70-14 knockout, hsp70-15 knockout and wild type plants was performed with leaf samples of 6 week old plants grown under ambient conditions. The analysis of differentially regulated genes compared to the wild type Col-0 plants exhibited a constitutive heat stress response / cytosolic protein response of Hsp70-15 deficient plants.

2011-03-28 | E-MEXP-3082 | biostudies-arrayexpress

Transcription profiling of Arabidopsis seedlings expressing LEC2-Glucocorticoid Receptor fusion protein, induced with dexamethasone for 0, 1 or 4 hours

Project description:LEAFY COTYLEDON2 activity was induced via a LEC2-Glucocorticoid Receptor fusion protein in Arabidopsis seedlings. Experiment Overall Design: Transgenic plants carrying a 35S:LEC2-Glucocorticoid Receptor transgene were used for LEC2 induction. Activity was induced in 8 day old seedlings for 1 or 4 hours with Dexamethasone application. Controls include uninduced transgenic plants and non-transgenic plants treated with Dex. Experiment Overall Design: Two biological replicates per treatment.

2007-10-03 | E-GEOD-3959 | biostudies-arrayexpress

Arabidopsis defensin, AtPDF1 protein detection

Project description:The goal of this project is to compare the amount of AtPDF1 protein in wild plants and amiRNA transgenic plants targeting the decrease of the 5 most similar AtPDF1 transcripts. Wild plants will be compared to two types of amiRNA transgenics, each expressing a different construct.

2023-06-26 | PXD037695 | Pride

Small RNA from Arabidopsis thaliana with RNA silencing directed to Chalcone Synthase (At5G13930) by a hairpin RNA, a 21-nt artificial miRNA and a 22-nt artificial miRNA in the pap1-D over-expressing background

Project description:22-nucleotide (nt) microRNAs (miRNAs) derived from asymmetric duplexes trigger trans-acting phased small interfering RNA (tphasiRNA) production from complementary targets. Here we investigate the efficacy of 22-nt artificial miRNA (amiRNA) mediated RNA silencing relative to conventional hairpin RNA (hpRNA) and 21-nt amiRNA mediated RNA silencing. CHALCONE SYNTHASE (CHS) was selected as a target in Arabidopsis thaliana due to the obvious and non-lethal loss of anthocyanin accumulation upon widespread RNA silencing. Over-expression of CHS in the pap1-D background facilitated visual detection of both local and systemic RNA silencing. RNA silencing was initiated in leaf tissues from hpRNA and amiRNA plant expression vectors under the control of an Arabidopsis RuBisCo small subunit 1A promoter (SSU). In this system, hpRNA expression triggered CHS silencing in most leaf tissues but not in roots or seed coats. Similarly, 21-nt amiRNA expression from symmetric miRNA/miRNA* duplexes triggered CHS silencing in all leaf tissues but not in roots or seed coats. However, 22-nt amiRNA expression from an asymmetric duplex triggered CHS silencing in all tissues, including roots and seed coats, in the majority of plant lines. This widespread CHS silencing required RNA DEPENDENT RNA POLYMERASE6 mediated accumulation of phasiRNAs from the endogenous CHS transcript. These results demonstrate the efficacy of asymmetric 22-nt amiRNA-directed RNA silencing and associated phasiRNA production and activity, in mediating widespread RNA silencing of an endogenous target gene. Asymmetric 22-nt amiRNA directed RNA silencing requires little modification of existing amiRNA technology and is expected to be effective in suppressing other genes and/or members of gene families. sRNA sequencing from aerial tissues of 3-week old plants grown on MS media

2013-10-14 | E-GEOD-49792 | biostudies-arrayexpress

Gene expression profiling in response to strigolactone signalling revealed by dexamethasone treatment to SL-insensitive version of SMXL7 tagged with glucocorticoid receptor (SMXL7d53-GR) in Arabidopsis hypocotyl.

Project description:Strigolactone (SL) signalling plays many roles in plants, however, the downstream responsive genes remain unclear. SUPPRESSOR OF MORE AXILLARY GROWTH2-LIKE 7 (SMXL7) is degraded upon SL signalling. Here, we generated Arabidopsis carrying a transgene SMXL7pro:SMXL7d53-GR, where a SL-insensitive version of SMXL7 is tagged with the ligand binding domain of the glucocorticoid receptor (GR). SMXL7d53-GR will be translocated to the nucleus upon dexamethasone (dex), and we profiled the gene expression in mock or dex treated plants and revealed the downstream responsive genes of SL signalling.

2024-07-01 | GSE270808 | GEO

Control of Reproductive Floral Organ Identity Specification in Arabidopsis by the C Function Regulator AGAMOUS (expression)

Project description:Characterization of the activities of the transcription factor that AG encodes throughout flower development using perturbation assays in combination with a floral induction system (FIS) that allows a stage-specific analysis of flower development. The series contains static perturbation experiments (null allele ag-1), and amiRNA knockdown experiments. Two conditions (i.e. perturbation experiments of ag-1 homozygous in the FIS vs ag-1 heterozygous in the FIS across three developmental stages. amiRNA knockdown experiments induced amiRNA expression vs. uninduced.

2013-07-01 | E-GEOD-44919 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data