NtrBC as a regulator of adaptive lifestyles and virulence
Ontology highlight
ABSTRACT:
ORGANISM(S): Pseudomonas aeruginosa
PROVIDER: GSE145591 | GEO | 2020/04/16
REPOSITORIES: GEO
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Project description:NtrBC as a regulator of adaptive lifestyles and virulence
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Project description:To compare the genome-wide transcriptional effect of ABA and iSB09 in tomato plants, we performed RNA-seq analysis of mock-, 10 uM ABA- or 20 uM iSB09-treated plants. Differential gene expression analysis between mock- and ABA-treated or iSB09-treated seedlings was done with DESeq2 and genes with an absolute value of log2 fold change (log2FC) > 1 or (log2FC) < -1 and p-adjusted value (padj) < 0.05 were selected. iSB09 upregulated and downregulated genes represent a subset of the ABA-responsive genes, which reflects the activation of PYL1-like and PYL4-like ABA receptors in tomato seedlings.
2024-07-22 | GSE256238 | GEO
Project description:Pyruvate kinase M2 (PKM2), as the terminal and last rate-limiting enzyme of the glycolytic pathway, is an ideal enzyme for regulating metabolic phenotype. PKM2 tetramer activation has shown a protective role against diabetic kidney disease (DKD). However, the molecular mechanisms involved in diabetic tubular has not been investigated so far. In this study, we performed transcriptome gene expression profiling in human renal proximal tubular epithelial cell line (HK-2 cells) treated with high D-glucose (HG) for 7 days before the addition of 10-μM TEPP-46, an activator of PKM2 tetramerization, for a further 1 day in the presence of HG. Afterwards, we analyzed the differentially expressed (DE) genes and investigated gene relationships based on weighted gene co-expression network analysis (WGCNA). The results showed that 2,902 DE genes were identified (adjusted P-value ≤ 0.05), where 2,509 DE genes (86.46%) were co-expressed in the key module. Four extremely down-regulated DE genes (HSPA8, HSPA2, HSPA1B and ARRB1) and three extremely up-regulated DE genes (GADD45A, IGFBP3 and SIAH1) enriched in the down-regulated endocytosis (hsa04144) and up-regulated p53 signaling pathway (hsa04115), respectively, were validated by the qRT-PCR experiments. The qRT-PCR results showed that the relative expression levels of HSPA8 (adjusted P-value = 4.45×10-34 & log2(FC) = -1.12), HSPA2 (adjusted P-value = 6.09×10-14 & log2(FC) = -1.27), HSPA1B (adjusted P-value = 1.14×10-11 & log2(FC) = -1.02) and ARRB1 (adjusted P-value = 2.60×10-5 & log2(FC) = -1.13) were significantly different (P-value < 0.05) from case group to control group. Furthermore, the interactions and predicted microRNAs of the key genes (HSPA8, HSPA2, HSPA1B and ARRB1) were visualized in networks. This study identified the key candidate transcriptomic biomarkers and biological pathways in the hyperglycemic HK-2 cells responding to the PKM2 activator TEPP-46. These results will be valuable for further research on PKM2 in DKD.
2022-08-29 | GSE205674 | GEO
Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare the transcriptomes of microbiota-specific memory CD4 T cells that were treated with vs without the combination of cell activation and metabolic checkpoint inhibition (CAMCI) strategy in vivo. Methods: mRNA profiles of donor-derived CBir1 TCR Tg CD4+ cells were generated by deep sequencing, with cells from 3-4 mice polling together per group, using Illumina HiSeq. The sequence reads that passed quality filters were analyzed with DESeq2 for gene expression comparisons between groups of samples. The Wald test was used to generate p-values and log2 fold changes. Results: Using an optimized data analysis workflow, we mapped about 50 million sequence reads per sample to the mouse genome (GRCm38). Depending on the groups that were compared, a range from 17-2868 transcripts showed differential expression, with an absolute log2 fold change >1 and an adjusted p value <0.01. Differentially expressed genes (DEGs) were used to generate a heatmap, a principle component analysis plot, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.
2020-11-12 | GSE161311 | GEO
Project description:rs04-02_ntr - ntr - Thioredoxins are small redox proteins implicated in crucial pathways of cell life. They are reduced by a conserved flavoprotein named NADPH-dependent thioredoxin reductase (NTR). In order to give an insight into the functions of the NTR/thioredoxin pathway in Arabidopsis, we have used a reverse genetic approch. Both NTR genes (NTRA and NTRB) have been inactivated in the double ntra ntrb mutant. T-DNA lines have been provided by the SALK library. Surprisingly, this mutant shows a limited phenotype suggested that some additional redox pathways are involved in compensating the inactivation of the NTR/thioredoxin pathway. In order to isolate compensatory genes that would be transcriptionally induced in the ntra ntrb mutant, we have performed this CATMA project. - Comparison of the transcript profile of the ntra ntrb knock-out mutant vs Col-0 wild-type ecotype. Seeds of the wild-type Col-0 ecotype and of the ntra ntrb homozygote double mutant were sowed in soil. Three replicates of lines were made (Col-0 1, 2 and 3 ntra ntrb 1, 2 and 3) : plants were put at different places of the greenhouse and grown at different times. Germination was synchronized by 4 days at 4degreeC and plants were grown during 17 days, until they reached a 6 rosette leaves stage. Entire plants (30 plants per sample) were harvested, frozen in liquid nitrogen and stored at 80degreeC until extraction. Keywords: gene knock out,wt vs mutant comparison
2007-06-20 | GSE7998 | GEO
Project description:rs04-02_ntr - ntr - Thioredoxins are small redox proteins implicated in crucial pathways of cell life. They are reduced by a conserved flavoprotein named NADPH-dependent thioredoxin reductase (NTR). In order to give an insight into the functions of the NTR/thioredoxin pathway in Arabidopsis, we have used a reverse genetic approch. Both NTR genes (NTRA and NTRB) have been inactivated in the double ntra ntrb mutant. T-DNA lines have been provided by the SALK library. Surprisingly, this mutant shows a limited phenotype suggested that some additional redox pathways are involved in compensating the inactivation of the NTR/thioredoxin pathway. In order to isolate compensatory genes that would be transcriptionally induced in the ntra ntrb mutant, we have performed this CATMA project. - Comparison of the transcript profile of the ntra ntrb knock-out mutant vs Col-0 wild-type ecotype. Seeds of the wild-type Col-0 ecotype and of the ntra ntrb homozygote double mutant were sowed in soil. Three replicates of lines were made (Col-0 1, 2 and 3 ntra ntrb 1, 2 and 3) : plants were put at different places of the greenhouse and grown at different times. Germination was synchronized by 4 days at 4degreeC and plants were grown during 17 days, until they reached a 6 rosette leaves stage. Entire plants (30 plants per sample) were harvested, frozen in liquid nitrogen and stored at 80degreeC until extraction. Keywords: gene knock out,wt vs mutant comparison 3 dye-swap - CATMA arrays
2007-06-19 | E-GEOD-7998 | biostudies-arrayexpress
Project description:This MS data set was used as validation data for an exploratory proteomics study of biomarkers in osteoarthritis (OA) using SOMAscan assay data. We analyzed human synovial fluid from three groups with the two methods: 1) deceased donors with mildly degenerated cartilage and/or meniscus, which is a well-known characteristic of OA, 2) deceased donors with no degeneration as controls and 3) total knee replacement patients with end-stage knee osteoarthritis. We conducted gaussian graphical network analyses of proteins from group 1 and 2 using SOMAscan assay data, and included the 800 aptamers/proteins with largest absolute log2 fold changes in the network construction. We found 102 differentially connected proteins, which of 31 were also present in the MS data set. After filtering out non-proteotypic peptides, proteins with only one peptide used for identification across all samples and proteins with more than 50% missing values, we ended up with 21 proteins. We analyzed these proteins by 1) estimating correlation between the methods on log2 standardized data sets and 2) compared log2 fold changes and 95%CIs by linear mixed effect model with quantification on the protein level as the outcome variable, adjusted for age and sex. Most proteins shown a positive correlation for the two methods. The comparison of log2 fold changes and 95%CIs between the methods show similar results for mild degeneration group when compared to healthy controls, whereas the results for the comparison between healthy controls and late stage OA group show dissimilarities for a few proteins.<br><br>Shared first authors: Martin Rydén, Amanda Sjögren<br>Authors: Patrik Önnerfjord, Aleksandra Turkiewicz, Jon Tjörnstrand*<br>Last authors: Neserin Ali (neserin.ali@med.lu.se), Martin Englund (martin.englund@med.lu.se)<br>Department of Clinical Sciences Lund, Lund University<br>*Department of Orthopedics, Skåne University Hospital, Sweden
2024-05-13 | PXD051182 | Pride
Project description:Analysis of abscisic acid (ABA) perception and signaling in crops is complicated by the multigenic nature of the PYR/PYL/RCAR ABA receptor family and the reported functional redundancy, which is a formidable challenge, particularly in polyploid plants. Therefore, few molecular genetics studies have been performed in crop species to study the contribution of ABA signaling to balance plant growth and adaptation to the environment. Our data reveal ABA-responsive genes in Nicotiana benthamiana, an allotetraploid biotech crop, and provide a molecular framework for gene expression analyses aimed at optimizing plant growth and environmental adaptation in agriculture. To obtain the genome-wide transcriptional effect of ABA in Nb WT plants, we performed RNA-seq studies as described in experimental procedures. Differential gene expression analysis between mock- and ABA-treated seedlings was done with DESeq2 and genes with an absolute value of log2 fold change (log2FC) > 1 or (log2FC) < -1 and p-adjusted value (padj) < 0.05 were selected
2024-08-13 | GSE255725 | GEO
Project description:Transcriptomic study of a thioredoxin reductase knock-out mutant.<br> <br> Biological question :<br> Thioredoxins are small redox proteins implicated in crucial pathways of cell life. They are reduced by a conserved flavoprotein named NADPH-dependent thioredoxin reductase (NTR). In order to give an insight into the functions of the NTR/thioredoxin pathway in Arabidopsis, we have used a reverse genetic approch. Both NTR genes (NTRA and NTRB) have been inactivated in the double ntra ntrb mutant. T-DNA lines have been provided by the SALK library. Surprisingly, this mutant shows a limited phenotype suggested that some additional redox pathways are involved in compensating the inactivation of the NTR/thioredoxin pathway. In order to isolate compensatory genes that would be transcriptionally induced in the ntra ntrb mutant, we have performed this CATMA project.<br> <br> Seeds of the wild-type Col-0 ecotype and of the ntra ntrb homozygote double mutant were sowed in soil. Three replicates of lines were made (Col-0 1, 2 and 3 ntra ntrb 1, 2 and 3) : plants were put at different places of the greenhouse and grown at different times. Germination was synchronized by 4 days at 4C and plants were grown during 17 days, until they reached a 6 rosette leaves stage. Entire plants (30 plants per sample) were harvested, frozen in liquid nitrogen and stored at -80C until extraction.<br> <br>
2005-09-15 | E-MEXP-365 | biostudies-arrayexpress
Project description:We compared the transcriptomic differences in rice leaves after being fed by C. medinalis using RNA sequencing. Global transcriptome changes in rice leaves responding to 1, 6, 12 and 24 h of such feeding were quantified by examining mRNAs of 20 libraries (four biological replicates at each of five sampling times) that collectively included 164.04 Gb of clean reads. Each of these samples contained at least 7.36 Gb of data with Q30 quality scores 90.70%. The GC content accounted for 53.16-53.71% of these reads. A total of 44.84-56.19 million reads were mapped uniquely to the rice IRGSP-1.0 reference genome, with unique mapping rates ranging from 89.73% to 91.36%. Using absolute value of log2(fold change) > 0 and FDR adjusted P value (padj) ≤ 0.05 as threshold for recognizing differentially expressed genes between any two treatments, a total of 6,372 DEGs were identified in rice leaves at different time points (1, 6, 12, and 24 h) of C. medinalis infestation.
2020-11-05 | GSE159259 | GEO