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Transcriptional analysis of tenocytes from mice with a targeted deletion of scleraxis

ABSTRACT: We report RNA sequencing data from tenocytes cultured from the tail tendons of adult male mice in the C57Bl/6 background that either have the scleraxis gene (Scx+) or mice in which scleraxis has been deleted using CreERT2 driven from the Rosa26 locus using 4-hydroxytamoxifen (4HT).

ORGANISM(S): Mus musculus

PROVIDER: GSE145863 | GEO | 2020/02/24

REPOSITORIES: GEO

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Transcriptional analysis of tendons from mice with a targeted deletion of scleraxis

Project description:We report RNA sequencing data from the plantaris tendons of adult male mice in the C57Bl/6 background that either have the scleraxis gene (Scx+) or mice in which scleraxis has been deleted using CreERT2 driven from the Rosa26 locus (Scx-). Mice in which scleraxis was deleted were created by crossing scleraxis-floxed mice with Rosa26-CreERT2 mice. Rosa26-CreERT2 mice that did not have a floxed scleraxis allele served as controls. Mice were treated with tamoxifen for 5 days to induce recombination at the scleraxis locus, and then subjected to a synergist ablation procedure in which the Achilles tendon is removed, resulting in compensatory growth of the plantaris tendon. Mice were analyzed either 7 or 14 days after synergist ablation. Control mice that did not undergo tamoxifen treatment or synergist ablation were also studied (NOC).

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