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Comparison of Regulatory Type of Macrophages and PCMO Cells from perspective of RNAseq data.

ABSTRACT: Gene expression plasticity is central for macrophages? timely responses to cues from the microenvironment permitting phenotypic adaptation from pro-inflammatory (M1) to wound healing and tissue-regenerative (M2, with several subclasses). Regulatory macrophages (Mreg) are a distinct macrophage type, partially sharing some functionalities with both M1 and M2 cells. Mreg possess immunoregulatory, anti-inflammatory, and angiogenic properties, and are considered as a potential allogeneic cell therapy product to treat clinical conditions, e.g., non-healing diabetic foot ulcers. In this study we characterized clinically relevant regulatory macrophages, Mreg and Mreg_UKR, programmable cells of monocytic origin (PCMO) and comparator macrophages (M1, M2a and M0) using flow-cytometry, RT-qPCR, phagocytosis and secretome measurements, and finally RNA-Seq. We demonstrate that conventional phenotyping has a limited potential in deciphering all classes of produced cells. This limitation was ameliorated when global transcriptome characterization by RNA-Seq was employed. Using this approach we prove that macrophage manufacturing processes can result in a highly reproducible cell phenotype. At the same time, minor changes introduced in a protocol can consistently effect the phenotype of the end product as well. Additionally, we have identified a novel constellation of potential process specific biomarkers, which will not only support further clinical product development, but will lead to a better understanding of macrophage biology, differentiation and polarized activation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE146028 | GEO | 2020/08/24

REPOSITORIES: GEO

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