Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Persistent Epigenetic Reprogramming of Sweet Taste by Diet

ABSTRACT: We report the application of ribosomal profiling based RNA sequencing technology for high-throughput profiling of the Gr5a cells of male Drosophila melanogaster adults. By expressing the UAS-Rpl3-3XFLAG transgene using the Gr5a-GAL4 driver on Pclc429 mutant flies.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE146241 | GEO | 2020/11/11

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Persistent Epigenetic Reprogramming of Sweet Taste by Diet

Project description:We report the application of ribosomal profiling based RNA sequencing technology for high-throughput profiling of the Gr5a cells of male Drosophila melanogaster adults. By expressing the UAS-Rpl3-3XFLAG transgene using the Gr5a-GAL4 driver.

2020-11-11 | GSE146214 | GEO

A nutrient information pathway links diet to taste (RNA-Seq)

2023-03-21 | GSE188756 | GEO

Persistent Epigenetic Reprogramming of Sweet Taste by Diet

Project description:We report the application of targeted DNA Adenine Methyltransferase identification DNA sequencing technology for high-throughput profiling of Pcl occupancy in the Gr5a cells of male Drosophila melanogaster adults. By expressing the UAS-LT3-Dam::Pcl and UAS-LT3-Dam transgene using the Gr5a-GAL4;tubulinGAL80ts driver.

2020-11-11 | GSE146242 | GEO

A nutrient information pathway links diet to taste (DNA-Seq II)

2023-03-21 | GSE188755 | GEO

A nutrient information pathway links diet to taste (DNA-Seq I)

Project description:We report the application of targeted DNA Adenine Methyltransferase identification DNA sequencing technology for high-throughput profiling of OGT occupancy in the Gr5a cells of male Drosophila melanogaster adults. By expressing the UAS-LT3-Dam::Pcl and UAS-LT3-Dam transgene using the Gr5a-GAL4;tubulinGAL80ts driver.

2023-03-21 | GSE188754 | GEO

Comparative analysis of gene expression changes upon overexpression of activated Thickveins in the Drosophila wing at larval vs. pupal stages

Project description:Investigation of whole genome gene expression changes in dissected Drosophila wings at the wandering L3 larval stage and 24h after pupa formation, expressing UAS-activated Thickveins Q-D under control of apterous-gal4 with a tubulin driven temperature sensitive gal80 transgene, compared to the parental strain lacking the UAS-Thickveins transgene

2012-07-04 | GSE39056 | GEO

Global quantitative proteomic analysis of aging Drosophila eyes

Project description:Wild-type Drosophila melanogaster expressing nuclear GFP-KASH fusion protein in photoreceptors for cell type-specific gene expression profiling (Rh1-Gal4>UAS-GFPKASH ; Genotype = w1118;; P{w+mC=[UAS-GFP-Msp300KASH}attP2, P{ry+t7.2=rh1-GAL4}3, ry506) were raised in 12:12h light:dark cycle at 25°C. Flies were aged for 10 or 40 days post-eclosion, and eyes were harvested from male flies for global quantitative proteomic analysis. Significantly changed proteins were identified that may contribute to age-associated retinal degeneration and loss of visual function in the aging Drosophila eye.

2021-07-23 | PXD027090 | Pride

Comparative analysis of gene expression changes upon Cabut or dE2F1/dDP overexpression in the Drosophila wing

Project description:Investigation of whole genome gene expression level changes in dissected Drosophila wings at the wandering L3 larval stage, 24h after pupa formation and 36h after pupa formation, expressing either UAS-Cabut or UAS-dE2F1 + UAS-dDP under control of apterous-gal4 with a tubulin driven temperature-sensitive gal80 transgene, compared to the parental strain lacking UAS transgenes.

2011-07-08 | GSE30484 | GEO

Senseless-responsive genes in larval salivary glands of late Drosophila prepupae

Project description:When misexpressed in late Drosophila prepupae, the transcription factor Senseless (Sens) blocks death of the larval salivary glands that normally occurs in the early pupa. The aim of the experiment was to identify genes responding to Sens that might mediate the effect of the protein on cell death and other biological processes. The yeast transcription factor GAL4, expressed from a heat-inducible transgene (P{GAL4-Hsp70.PB}89-2-1), was used to drive expression of Sens from a UAS-sens transgene. After crossing the GAL4 and UAS lines, expression of GAL4 was induced by a 30-min heat shock treatment (37 °C) of the progeny at 9 hours after puparium formation. Salivary glands were dissected at 14 hours after puparium formation and RNA isolated for microarray analysis with Affymetrix GeneChips. Control samples were obtained from animals treated the same way carrying one copy of the GAL4 transgene (progeny of a cross between flies of the P{GAL4-Hsp70.PB}89-2-1 and w1118 strains) and w1118 animals. The microarray data identified several genes associated with programmed cell death, including caspase genes, which respond to Sens. In addition, the data show that many Drosophila genes respond to the yeast transcription factor GAL4 in a UAS-independent manner. To identify target genes of Sens that are of biological relevance, gene expression patterns in the presence of Sens were compared to gene expression patterns in both the presence and the absence of GAL4. This comparison revealed that Sens seems to preferentially downregulate targets that are upregulated by GAL4, suggesting that these genes may not necessarily constitute true transcriptional targets of Sens. Keywords: ectopic expression experiment

2008-05-29 | GSE8619 | GEO

Persistent Epigenetic Reprogramming of Sweet Taste by Diet

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-11-11 | GSE146245 | GEO