Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from control and Angpt2 silenced mouse melanoma cells

ABSTRACT: The functional role of tumor cell-expressed Angpt2 still remains elusive. Here, we used mouse melanoma cells which have endgeneous Angpt2 expression and invesitgated the functional role of tumor cell-derived Angpt2. Total RNA from control and Angpt2 silenced mouse melanoma cells (RET) was isolated and was further utilized for microarray analysis using Affymetrix Mouse Gene 2.0 ST array.

ORGANISM(S): Mus musculus

PROVIDER: GSE146320 | GEO | 2020/04/30

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Expression data from control and Angpt2 silenced mouse melanoma cells

Project description:Expression data from control and Angpt2 silenced mouse melanoma cells

| PRJNA610074 | ENA

Gene expression profiles of human mesangial cell line treated with and without ANGPT2

Project description:Glomerular diseases are the leading cause of chronic kidney diseases with pathomechanisms largely unclear. It is known that ANGPT2 regulates endothelial cell homeostasis and function via TEK/TIE2 and its deregulation causes endothelial damage. We found that ANGPT2 is upregulated in glomerular diseases and wondered whether it has any effect on glomerular podocytes and mesangial cells given that they have no or low TEK expression. We treated podocytes and mesangial cells in culture with ANGPT2 and found no overt cellular changes. RNA-seq analysis showed that gene expression was altered in both podocytes and mesangial cells and that the regulated genes in the two cell types were fundamentally different. GO and KEGG analyses showed that the two groups of regulated genes were enriched in distinct processes and pathways. These results suggest that ANGPT2 exerts effects on both podocytes and mesangial cells and that increased ANGPT2 may be involved in glomerular injury by affecting podocytes and mesangial cells in addition to endothelial cells.

2022-01-10 | GSE185293 | GEO

Gene expression profiles of human podocyte cell line treated with and without ANGPT2

2022-01-10 | GSE185292 | GEO

Quantitative proteomics of CELF1-silenced human melanoma cell lines

Project description:CELF1 was silenced in two human melanoma cell lines (SKMEL-103 and UACC-62, indicated as 9M and 17M, respectively) using short hairpin RNA (tag 114). As control scrambled non targeting shControl transduced cell were used (tag 115).

2017-12-21 | PXD003112 | Pride

Conduct gene profiling experiment from a melanoma cell line which silenced galectin-3 gene expression to understand different phenotypic properties observed during tumor growth

Project description:Conduct gene profiling experiment from a melanoma cell line which silenced galectin-3 gene expression to understand different phenotypic properties observed during tumor growth. A melanoma cell line which silenced galectin-3 gene expression was obtained. This cell line was used in overexpression experiments, using a galectin-3 coding plasmid. Both galectin-3 negative and positive cells were obtained in syngeneic wild type and galectin-3 knockout mice. Altogether, we had evidence that while galectin-3 derived from the tumor cells impaired tumor growth, galectin-3 derived from stromal cells apparently favored tumor growth. Angiogenic response within tumors derived from galectin-3 expressing melanoma cells was delayed as compared to control conditions. Cells recruited to these distinct microenvironments seem to control tumor growth. Gene profiling of this tissue will allow for an integrative view of the process that was somehow orchestrated by galectin-3.

2011-09-05 | E-GEOD-31922 | biostudies-arrayexpress

Conduct gene profiling experiment from a melanoma cell line which silenced galectin-3 gene expression to understand different phenotypic properties observed during tumor growth

2011-09-06 | GSE31922 | GEO

mRNA expression data for VPS39-silenced human muscle cells and controls

Project description:mRNA expression data for VPS39-silenced human muscle cells and controls (n=6 per group) were obtained using GeneChip™ Human Gene 2.0 ST Assay (Applied Biosystems)

2020-12-14 | GSE157345 | GEO

ERK1/2 controlled genes ANGPT2 and CXCR4 mediate liver metastasis from colon cancer

Project description:Carcinoma development in colorectal cancer (CRC) is driven by genetic alterations in numerous signaling pathways. Alterations in the RAS-ERK1/2 pathway are associated with the shortest overall survival for patients after diagnosis of CRC metastatic disease, but how RAS-ERK signaling regulates CRC metastasis is still unknown. An unbiased screening approach based on selection of highly liver metastatic CRC cells in vivo was used to determine genes associated with metastasis and a ERK1/2-controlled metastatic gene set (EMGS) defined. The ANGPT2 and CXCR4, two genes within the gene set, were subjected to gain and loss of function validation in CRC cells (SW620, SW480, Colo26, HCT116), its downstream mechanism validated, and tested in clinical samples. Here, we identified an EMGS that is associated with increased recurrence and reduced survival for patients with CRC tumors. Higher levels of EMGS expression were detected in the CRC subsets Consensus Molecular Subtype (CMS)1 and CMS4. We show that the RAS-ERK1/2 axis controls the expression of the cytokine ANGPT2 and the cytokine receptor CXCR4 in CRC cells, and that this facilitates the development of liver but not lung metastases, suggesting that ANGPT2 and CXCR4 are essential for metastatic outgrowth in the liver. We also show that CXCR4 also controls the expression of the cytokines IL10 and CXCL1, and provide evidence for a causal role of IL10 in supporting liver colonization. In summary, we demonstrate that amplification of ERK1/2 signaling in KRAS-mutated CRC cells affects the cytokine milieu of the tumors, thus possibly affecting tumor?stroma interactions and favoring liver metastasis formation.

2020-07-31 | GSE142219 | GEO

Beta-catenin Controls Metastasis in Braf-activated Pten-inactivated Melanomas

Project description:Malignant melanoma is characterized by frequent metastasis, however specific changes that regulate this process have not been clearly delineated. Although it is well known that Wnt signaling is frequently dysregulated in melanoma, the functional implications of this observation are unclear. By modulating beta-catenin levels in a mouse model of melanoma that is based on melanocyte-specific Pten loss and BrafV600E mutation, we demonstrate that beta-catenin is a central mediator of melanoma metastasis to lymph node and lung. In addition to altering metastasis, beta-catenin levels control tumor differentiation and regulate both MAPK/Erk and PI3K/Akt signaling. Highly metastatic tumors with beta-catenin stabilization are very similar to a subset of human melanomas; together these findings establish Wnt signaling as a metastasis regulator in melanoma. MoGene-1_0-st-v1: Four samples total. Two biological replicates of uncultured Pten/Braf murine melanomas and two biological replicates of uncultured Pten/Braf/Bcat-STA murine melanomas. MoEx-1_0-st-v1: Two samples total. Dissociated tumor and FACS-enriched Pten/Braf and Pten/Braf/Bcat-STA murine melanoma.

2011-10-12 | E-GEOD-32907 | biostudies-arrayexpress

Genome-wide maps of p-STAT3 in PD-L1 silenced uveal melanoma cells and control cells

Project description:We performed CUT&Tag with anti-p-STAT3 antibodies in PD-L1 silenced uveal melanoma cells and control cells

2023-04-04 | GSE202883 | GEO