Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Signalling networks constructed by integrative analysis of multi-omics in peripheral blood mononuclear cells in systemic lupus erythematous patients

ABSTRACT: Omics technologies and biomolecules included genomics (DNA), whole transcriptomics (miRNAs, lncRNAs, circRNAs and mRNAs), proteomics (proteins) were used for collecting high throughput data from peripheral blood mononuclear cells (PBMCs) of subjects in our study. The joint analysis method of multi-omics was taken advantage of in our studies for uncovering some SLE disease-related molecular regulatory networks. For WGBS study, PBMCs of blood samples from the people recruited by our study were collected and used for whole-genome bisulphite sequencing (WGBS). There were 10322 genes presenting differential methylation levels in PBMCs of SLE patients (6305 hypermethylation genes and 4017 hypomethylation genes).

ORGANISM(S): Homo sapiens

PROVIDER: GSE146410 | GEO | 2021/06/11

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Splinted Ligation Adapter Tagging, a novel library preparation for whole genome bisulphite sequencing

Project description:Library preparation for whole genome bisulphite sequencing (WGBS) is challenging due to side effects of the bisulphite treatment, which leads to extensive DNA damage. Recently, a new generation of methods for bisulphite sequencing library preparation have been devised. They are based on initial bisulphite treatment of the DNA, followed by adaptor tagging of single stranded DNA fragments, and enable WGBS using low quantities of input DNA. In this study, we present a novel approach for quick and cost effective WGBS library preparation that is based on splinted adaptor tagging (SPLAT) of bisulphite-converted single-stranded DNA. Moreover, we validate SPLAT against three commercially available WGBS library preparation techniques, two of which are based on bisulphite treatment prior to adaptor tagging and one is a conventional WGBS method.

2016-10-27 | GSE89213 | GEO

Cellular spermine targets JAK1 to restrain cytokine-mediated autoimmunity (Human)

Project description:Whole blood samples from SLE patients were collected and PBMCs were isolated using Ficoll-PaqueTM PLUS reagent according to the manufacturer’s protocol. The fresh isolated PBMCs from SLE individuals were treated with DMSO or spermine for 3h, PBMCs from healthy control were used as control, then samples were harvested and lysated with Trizol reagend for RNA-sequencing analysis.

2024-06-21 | GSE223097 | GEO

RNA samples of human peripheral blood mononuclear cells for Methylated RNA immunoprecipitation-seq

Project description:We established m6A modification profiles using MeRIP-seq in peripheral blood mononuclear cells (PBMCs) from systemic lupus erythematosus (SLE) patients and controls (HC), and investigated m6A-related lncRNAs in SLE for novel potential roles in SLE. Compared with controls, m6A level was lower in SLE patients,426 lncRNAs and 2,331 mRNAs were differentially expressed in SLE patients.

2023-01-31 | GSE173312 | GEO

Transcriptome Analysis of PBMCs in peripheral blood of patients with SLE

Project description:The aim of this study was to analyze the expression profile of circRNAs in PBMCs in peripheral blood of patients with SLE by next-generation sequencing, and to identify potential markers for early diagnosis and prognosis of SLE, and to lay a foundation for further study on the mechanism of this molecule in PBMCs.

2021-07-14 | GSE169080 | GEO

Investigation of gene expression profiles in CD4+ T cells from SLE patients and normal controls

Project description:To investigate the lncRNAs expression profiling in CD4+ T cells of systemic lupus erythematosus (SLE) patients, we have employed “Agilent Human lncRNA 4*180K microarray” as a discovery platform to identify lncRNAs and mRNAs expression signatures in CD4+ T cells between SLE patients and normal controls. CD4+ T cells were isolated from peripheral blood mononuclear cells (PBMCs) of peripheral blood in SLE patients and normal controls, respectively.

2021-08-05 | GSE181500 | GEO

Expression data in PBMCs from SLE patients and controls

Project description:Peripheral blood mononuclear cells were collected from SLE patients in an observational study performed at the University of Michigan Blood microarray expression data were used to confirm the presence of an Interferon signature and identify additional surrogate genes RNA from PBMCs of SLE patients and normal donor controls were obtained for one time point and were profiled on Affymetrix arrays

2015-03-31 | E-GEOD-50772 | biostudies-arrayexpress

Molecular mechanisms of Target organ damage in youth with primary hypertension [bulk RNA-Seq 1]

Project description:High blood pressure or Primary Hypertension is associated with target organ damage (TOD) in both adults and youth, where TOD predicts the adverse cardiovscular (CV) events. The mechanisms involved in blood pressure mediated TOD are limitedly understood in onset of TOD. To understand this, first using RNA sequencing, we identified candidate differentially expressed genes in circulating PBMCs of study participants with and without hypertension and elevated left ventrivlar mass index . Next, through miRNA and whole genome bisulphite sequencing of circulating PBMCs we determined the epigenetic regulators of TOD. Also, using LCMS, serum proteomics, the key circulatory protein involved in advancing CV- TOD events was identified. In conclusion, multiomics approach was utilized to define the circulatory regulators involved in BP mediated CV-TOD.

2024-07-31 | GSE262828 | GEO

Molecular mechanisms of Target organ damage in youth with primary hypertension [smallRNA-Seq 1]

2024-07-31 | GSE262818 | GEO

Molecular mechanisms of Target organ damage in youth with primary hypertension [bulk RNA-Seq 2]

2024-07-31 | GSE262814 | GEO

Molecular mechanisms of Target organ damage in youth with primary hypertension [smallRNA-Seq 2]

2024-07-31 | GSE262819 | GEO