Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Stimulation of Caco-2 cells with IFNg

ABSTRACT: Caco-2 cells (intestinal human derived cell line) were cultured on transwell plates, and exposed to a stimulus (IFNg) through the bottom compartment of the transwell plates

ORGANISM(S): Homo sapiens

PROVIDER: GSE146893 | GEO | 2021/02/16

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Stimulation of Caco-2 cells with IFNg

Project description:Stimulation of Caco-2 cells with IFNg

| PRJNA612310 | ENA

Effects of Bifidobacterium bifidum PRL2010 on gene expression in intestinal epithelial cells

Project description:Analysis of gene expression in Caco-2 intestinal epithelial cells stimulated with Bifidobacterium bifidum PRL2010. We used microarrays to investigate gene expression in intestinal epithelial cells in response to Bifidobacterium bifidum PRL2010, in particular genes involved in mucin pathways. Caco-2 cells were grown in transwell plates to 4 days post-confluence. Cells were then incubated for 2h and 4h with Bifidobacterium bifidum PRL2010. The experiment was performed in duplicate. Caco-2 RNA was extracted and hybridized to Affymetrix NuGO_Hs1a52018 arrays.

2010-10-01 | E-GEOD-21976 | biostudies-arrayexpress

IFNg+ vs IFNg- Treg

Project description:Gene expression studies comparing IFNg+ Tregs versus IFNg- Tregs from human peripheral blood Ex vivo sorted Tregs (CD25highCD127neg) were stimulated for 4 hours and IFNg-secreting cells were detected by a IFNg-capture kit. The samples were resorted based on IFNg expression.

2016-07-01 | E-GEOD-71274 | biostudies-arrayexpress

Transcription profiling of CaCo-2 cells differentiated to M cells - establishing and characterizing a cell culture model for M cells

Project description:M cells are special cells in the epithelium of the small intestinal bowel and play an important role during the initiation of a primary, fast immune response. On a Costar-Transwell filter cells of a colon adeno carcinoma cell line (Caco-2) are growing until differentiation. Then on the other side of the Caco cell layer lymphocytes from human venous blood are added. Pores in the filter membrane allow contact between lymphocytes and Caco cells. Therefore some cells of the Caco cell layer are differentiating to "M cell like" cells, showing the morpholgy and function of natural M cells. Experiment Overall Design: Two replicates of non-induced filter-grown Caco cells and three replicates of lymphocyte-induced filter-grown Caco cells (including lymphocyte expression) for the identification of regulated genes; one experiment of bottle-grown Caco cells and one experiment of bottle-grown lymphocytes for baseline expression profiling and control reasons.

2008-06-12 | E-GEOD-3054 | biostudies-arrayexpress

Caco-2 cocultured with THP-1, time course

Project description:Previously, we constructed a coculture model to analyze the effect of macrophages on intestinal epithelial cells, and found that TNF-a secreted from human macrophage-like THP-1 cells induced cell damage to intestinal epithelial Caco-2 cells (Exp.Cell.Res. 2006, 312(19):3909-19). In this study, we present activation of NF-kB in Caco-2 cells within 15 min after coculturing. To reveal how TNF-a secreted from THP-1 cells affects Caco-2 cells in an early stage of coculture, we exhaustively analyzed the changes of gene expression in Caco-2 cells cocultured with THP-1 cells over the time periods of 0, 1, 3, 6, 24, and 48 h by using a DNA microarray. Differentially expressed genes extracted with maSigPro demonstrated that IEX-1 was the lowest p-value gene, that is, the most significantly changed gene among the up-regulated genes. The genes expressed in a similar pattern to IEX-1 involved immunity, apoptosis, and protein kinase cascade. These findings suggest that the stimuli of TNF-a from THP-1 cells activates NF-kB, leading induction of various gene expression. This pattern of gene expression indicates that not only early defense response but also cell death occurs at the same time, causing inflammatory condition. Caco-2 cells were cultured for 14 days on a semi-permeable support, and THP-1 cells were differentiated with phorbol myristate acetate (PMA) for 4 days in 12-well plates. Then, the semi-permeable support membrane in which Caco-2 cells had been cultured was placed on the 12-well plates on which THP-1 has been cultured.

2010-04-06 | E-GEOD-17625 | biostudies-arrayexpress

IFNg+ vs IFNg- Treg

Project description:Gene expression studies comparing IFNg+ Tregs versus IFNg- Tregs from human peripheral blood

2016-07-01 | GSE71274 | GEO

miRNA expression screening in Liver X Receptor (NR1H3) agonist (GW3965) -treated differentiated Caco-2/TC7 cells

Project description:Differentiated human enterocytic Caco-2/TC7 cells grown on transwell were incubated with LXR agonist GW3965 (5µM) or Vehicle DMSO (1:2000 ; vol:vol) for 48 hours. Total RNA were extracted using mirVana miRNA Isolation Kit (life technologies) then processed with Flash Tag Biotin HSR kit (Affymetrix) and hybridized on an Affymetrix GeneChip miRNA 4.0 Array.

2016-06-03 | E-MTAB-3675 | biostudies-arrayexpress

Differential gene expression in IFNg+ and IFNg- IL-15 primed NK cells after anti-NK1.1 stimulation [IFNgReporter_RNA-seq]

Project description:We isolated splenic NK cells and cultured them for 3 days in 100 ng/ml IL-15. Next, we stimulated the cells with plate-bound anti-NK1.1 for 6 hours and sorted YFP+ (IFNg+) and YFP- (IFNg-) populations. We then performed gene expression profiling analysis using data obtained from RNA-seq from unstimulated, YFP+ or YFP- NK cells.

2023-08-01 | GSE227863 | GEO

Interferon-gamma timecourse in IFNg-/- and SOCS1-/-IFNg-/- mouse livers

Project description:Timecourse analysis of Interferon-Gamma (IFNg) signalling in mice deficient for IFNg or both IFNg and Suppressor of Cytokine Signalling-1 (SOCS1). Keywords: time course expression analysis

2006-02-14 | GSE4232 | GEO

Transcriptomics effects of IFNb and IFNg stimulation on murine cancer cell lines

Project description:To measure the transcriptomic changes in response to IFNb and IFNg stimulation, we collected RNAseq of various cell lines that were stimulated or unstimulated by interferon for 48 hours

2022-08-01 | GSE206443 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data