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Single-molecule regulatory architectures captured by chromatin fiber sequencing [Hs Fiber-seq]

ABSTRACT: We report a method for precisely stenciling the structure of individual chromatin fibers onto their composite DNA templates using non-specific DNA N6-adenine methyltransferases. Single-molecule long-read sequencing using PacBio of these chromatin stencils enables nucleotide-resolution readout of the primary architecture of multi-kilobase chromatin fibers (Fiber-seq).

ORGANISM(S): Homo sapiens

PROVIDER: GSE146941 | GEO | 2020/06/25

REPOSITORIES: GEO

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