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Transcriptional profiling of purified endocardial and coronary vascular endothelial cells, isolated from murine embryonic hearts, at two developmental stages

ABSTRACT: Given that the Nes-gfp allele specifically labels coronary ECs, endogenous GFP and the endomucin marker (which was highly expressed in endocardial cells) were used together to separate endocardial and coronary vascular endothelial cell subpopulations in different developmental stages

ORGANISM(S): Mus musculus

PROVIDER: GSE147117 | GEO | 2021/02/20

REPOSITORIES: GEO

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Transcriptional profiling of endocardial and (arterial or venous-enriched) coronary vascular endothelial cells, purified from murine embryonic hearts at E17.5.

Project description:A specific tracing system, selectively labeling tomato+ intramyocardial vessels with the Nes-CreER driver, was used together with the Nes-Gfp reporter and endomucin marker to separate, from the same hearts, the three subsets of Ecs (ventricular endocardium, intramyocardial arterial-enriched, and subepicardial venous-enriched endothelial cells), which were subject to transcriptome profiling by RNASeq.

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Transcriptional profiling of purified endocardial and coronary vascular endothelial cells, isolated from murine embryonic hearts, at two developmental stages

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Transcriptional profiling of endocardial and (arterial or venous-enriched) coronary vascular endothelial cells, purified from murine embryonic hearts at E17.5.

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