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RNA-seq analysis of the transfer of R388 plasmid with or without ardC, from E. coli to P. putida

ABSTRACT: P. putida KT2440 was mixed in a conjugation filter with either E. coli BW27783-NxR bearing no plasmid (NP), E. coli BW27783-NxR bearing pSU2007 (ardC+) or E. coli BW27783-NxR bearing pLGM25 (ardC-). RNA-seq results showed that: (a) R388 genes involved in conjugation are highly upregulated in the ardC+ condition with respect to the ardC- condition. (b) a number of donor E. coli genes and pathways involved in flagellar motility, SOS and stress responses and different metabolic pathways are downregulated in the ardC- condition with respect to NP or ardC+ conditions. (c) SOS genes are upregulated in recipient P. putida cells when receiving ardC+ containing plasmid with respect to NP or ardC- conditions.

ORGANISM(S): Pseudomonas putida KT2440 Escherichia coli

PROVIDER: GSE147336 | GEO | 2020/03/22

REPOSITORIES: GEO

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