Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

L. lactis cultures: control vs acetaldehyde-induced

ABSTRACT: Transcriptome analysis of Lactococcus lactis cultures comparing control non-induced and induced with acetaldehyde

ORGANISM(S): Lactococcus lactis Lactococcus cremoris

PROVIDER: GSE147696 | GEO | 2020/03/30

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

L. lactis cultures: control vs diacetyl-induced

Project description:Transcriptome analysis of Lactococcus lactis cultures comparing control non-induced and induced with diacetyl

2020-03-30 | GSE147695 | GEO

L. lactis cultures: control vs acetaldehyde-induced

Project description:L. lactis cultures: control vs acetaldehyde-induced

| PRJNA616125 | ENA

L. lactis cultures: control vs diacetyl-induced

Project description:L. lactis cultures: control vs diacetyl-induced

| PRJNA616126 | ENA

Lactococcus lactis MG1363 ∆guaA versus L. lactis MG1363 (wt)

Project description:Gene expression in Lactococcus lactis MG1363 was compared to that of L. lactis MG1363 ∆guaA in rich GM17 medium.

2016-01-12 | GSE76764 | GEO

Gene expression analysis of MCF12A human breast cultures exposed to ethanol or acetaldehyde [4 weeks]

Project description:Alcoholism is associated with breast cancer incidence and progression, and moderate chronic consumption of ethanol is a risk factor. The mechanisms involved in alcohol's oncogenic effects are unknown, but it has been speculated that they may be mediated by acetaldehyde. Here, we use the immortalized normal human epithelial breast cell line MCF-12A to determine whether short- or long-term exposure to ethanol or to acetaldehyde, using in vivo compatible ethanol concentrations, induces their oncogenic transformation and/or the acquisition of epithelial mesenchymal transition (EMT). Cultures of MCF-12A cells were incubated with 25 mM ethanol or 2.5 mM acetaldehyde for 1 week, or with lower concentrations (1.0-2.5 mM for ethanol, 1.0 mM for acetaldehyde) for 4 weeks. In the 4 wk incubation, cells were also tested for anchorage independence, including isolation of soft agar selected cells (SASC) from the 2.5 mM ethanol incubations. Cells were analyzed by immuno-cytofluorescence, flow cytometry, western blotting, DNA microarrays, RT/PCR, and assays for miRs. We found that short-term exposure to ethanol, but not, in general, to acetaldehyde, was associated with transcriptional upregulation of the metallothionein family genes, alcohol metabolism genes, and genes suggesting the initiation of EMT, but without related phenotypic changes. Long-term exposure to the lower concentrations of ethanol or acetaldehyde induced frank EMT changes in the monolayer cultures and in SASC as demonstrated by changes in cellular phenotype and mRNA expression. This suggests that low concentrations of ethanol, with little or no mediation by acetaldehyde, induce EMT and some traits of oncogenic transformation such as anchorage independence in normal breast epithelial cells. MCF12A monolayer cultures were exposed to ethanol or acetaldehyde for either 1 week or 4 weeks. Total RNA samples were assayed for gene expression and for microRNA expression.

2017-02-10 | GSE76951 | GEO

Gene expression analysis of MCF12A human breast cultures exposed to ethanol or acetaldehyde [1 week]

2017-02-10 | GSE76950 | GEO

Gene expression analysis of MCF12A human breast cultures exposed to ethanol or acetaldehyde [miRNA]

Project description:MCF12A monolayer cultures were exposed to ethanol or acetaldehyde for eiher 1 week or 4 weeks Total RNA samples were assayed for gene expression and for microRNA expression. MicroRNA expression was accomplished using 2 experiments. The first experiment included untreated control, ethanol treated (4 weeks at 2.5 mM) and acetaldehyde treated (4 weeks at 1 mM). The data are presented in order of control1 for the ethanol and acetaldehyde treated samples followed by the ethanol and acetaldehyde treated samples, and then the control2 for the soft agar selected sample followed by the soft agar selected sample. The microRNA names are included in separate columns.

2017-02-10 | GSE76952 | GEO

Target identification of the cold stress induced sRNA CisR in Lactococcus lactis

Project description:Target identification of the cold stress induced sRNA CisR in Lactococcus lactis by CisR overexpression and MAPS technology

2018-06-19 | GSE115974 | GEO

Lactococcus lactis MG1363 â??guaA versus L. lactis MG1363 (wt)

Project description:Gene expression in Lactococcus lactis MG1363 was compared to that of L. lactis MG1363 â??guaA in rich GM17 medium. One condition design comparison of two strains

2016-01-12 | E-GEOD-76764 | biostudies-arrayexpress

Identification of tRNA sequences in Lactococcus lactis

Project description:we report the identification and sequences of the tRNAome of industrially relevant microorganism Lactococcus lactis

2014-07-29 | GSE55392 | GEO