Project description:The Arabidopsis thaliana ATH1 encodes a BEL1-like TALE homeodomain (BLH) transcription factor that controls the development of shoot organ boundaries. As part of a screen for genes that mediate the function of ATH1 in shoot development, we performed ChIP-seq to identify genome-wide ATH1 binding sites in developing seedlings.
Project description:We found that histone acetylation relays positional information and that a mutant altered in the histone deacetylase (HDAC) gene family member HDA18 exhibits altered H and N epidermal cell patterning. We then performed genome-wide location (ChIP-chip) analyses by using antibody against HDA18 in transgenic HDA18 overexpression Arabidopsis plants. These analyses identified statistically significant enriched DNA associated with HDA18. A total of 286 DNA fragments were enriched as putative HDA18 protein-binding sites.
Project description:Genome-wide analysis of CHD8 binding sites as well as sites associated with the activating histone marks 2mk4H3 and 3mK4H3. These analyses have been performed by ChIP-chip using a custom microarray containing promoter regions and CpG islands. ChIP experiments were performed with the C33A human cervix carcinoma cell line.
