Genomics

Dataset Information

0

HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription [ATAC-Seq]


ABSTRACT: Functional crosstalk between histone modifications and chromatin remodeling has emerged as a key regulatory mode of transcriptional control during cell fate decisions, but the underlying mechanisms are not fully understood. Here we demonstrate that HRP2-DPF3a-BAF complex coordinates histone H3 lysine 36 methylation (H3K36me) and ATP-dependent chromatin remodeling to regulate chromatin dynamic and gene transcription during myogenic differentiation. Mechanistically, through its HIV integrase binding domain (IBD), HRP2 associates with BRG1/BRM associated factor (BAF) chromatin remodeling complex by direct interaction with BAF45c (DPF3a) subunit. Through its Pro-Trp-Trp-Pro (PWWP) domain, HRP2 preferentially binds to H3K36me2. Integrative transcriptomic and cistromic analyses, coupled with ATAC-seq, reveal that HRP2 and DPF3a activate myogenic genes by increasing chromatin accessibility through recruitment of BRG1, the ATPase subunit of the BAF complex.

ORGANISM(S): Mus musculus

PROVIDER: GSE148296 | GEO | 2020/05/13

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2020-05-13 | GSE148295 | GEO
2020-05-13 | GSE148294 | GEO
| PRJNA593297 | ENA
| PRJNA623794 | ENA
| PRJNA623795 | ENA
| PRJNA623796 | ENA
2019-11-13 | PXD012721 | Pride
2010-12-22 | E-GEOD-26191 | biostudies-arrayexpress
2013-03-30 | E-GEOD-45625 | biostudies-arrayexpress
2020-12-23 | PXD018381 | Pride