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Pig brain miRNA profiling

ABSTRACT: Purpose: The aim of present research was to characterize miRNA profile of the pig brain tissue and identify miRNAs potentially connected with brain functioning. Methods: miRNA-seq analysis was performed on brain samples collected from 21 male and female pigs belonging to the Polish 990 synthetic line. The miRNA libraries were constructed from total RNA using NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) according to the manufacturer protocol. The quantification of the obtained libraries was performed on a Qubit 2.0 spectrophotometer (Invitrogen, Life Technologies), while a quality control on a TapeStation 2200 instrument (D1000 ScreenTape; Agilent). 100 single-end cycle sequencing was performed on the HiScanSQ platform (Illumina) with the use of TruSeq SR Cluster Kit v3- CBOT-HS and TruSeq SBS Kit v 3 - HS (Illumina). MicroRNA differentially expressed between males and females were identified with the DESeq2 software, while validation was carried out with RT-qPCR. Results: miRNA-seq approach allowed the identification of 237 known and 286 potentially novel miRNAs. The comparison of miRNA profiles between females and males showed differential expression of 38 microRNAs. Pathway analysis (DIANA mirPath v.3 tool) confirmed that detected miRNAs are engaged in estrogen signaling pathway, prolactin signaling pathway, long-term depression, axon guidance according to KEGG Database, and response to stress, immune system process, catabolic process according to GO. Conclusions: Obtained results enabled us to characterize the miRNA profile of the pig brain tissue of males and females, with particular emphasis on the influence of sex. Moreover, they can be the basis for future studies in terms of search of candidate miRNAs related with important processes occuring in the brain and its diseases.

ORGANISM(S): Sus scrofa

PROVIDER: GSE148302 | GEO | 2020/12/01

REPOSITORIES: GEO

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Pig brain miRNA profiling

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