Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome wide status of endometrial stromal cells stimulated by cAMP, and cAMP under C/EBPb-knockdown

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE148622 | GEO | 2021/02/17

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Genome wide H3K27ac status of endometrial stromal cells stimulated by cAMP, and cAMP under C/EBPb-knockdown

Project description:To know the role of C/EBPb in the changes of H3K27ac during desidualization, we compared the H3K27 profiles between cAMP-stimulation and cAMP-stimulation under C/EBPb-knockdown.

2021-02-17 | GSE148620 | GEO

Genome wide RNA expression profile of endometrial stromal cells stimulated by cAMP, and cAMP under C/EBPb-knockdown

Project description:To know the role of C/EBPb in the change of mRNA expression during desidualization, we compared the mRNA expression profiles between cAMP-stimulation and cAMP-stimulation under C/EBPb-knockdown.

2021-02-17 | GSE148621 | GEO

Genome wide status of endometrial stromal cells stimulated by cAMP, and cAMP under C/EBPb-knockdown

Project description:Genome wide status of endometrial stromal cells stimulated by cAMP, and cAMP under C/EBPb-knockdown

| PRJNA625210 | ENA

Genome wide H3K27ac status of endometrial stromal cells stimulated by cAMP, and cAMP under C/EBPb-knockdown

Project description:Genome wide H3K27ac status of endometrial stromal cells stimulated by cAMP, and cAMP under C/EBPb-knockdown

| PRJNA625204 | ENA

Genome wide RNA expression profile of endometrial stromal cells stimulated by cAMP, and cAMP under PGC-1a-knockdown

Project description:To know the role of PGC-1a in the change of mRNA expression during desidualization, we compared the mRNA expression profiles between cAMP-stimulation and cAMP-stimulation under PGC-1a-knockdown.

2022-05-04 | GSE167990 | GEO

Genome wide RNA expression profile of endometrial stromal cells stimulated by cAMP, and cAMP under C/EBPb-knockdown

Project description:Genome wide RNA expression profile of endometrial stromal cells stimulated by cAMP, and cAMP under C/EBPb-knockdown

| PRJNA625218 | ENA

Genome wide RNA expression profiles of endometrial stromal cells stimulated by cAMP, cAMP+MPA, MPA and E2+MPA.

Project description:To know the differences of decidualization induced by different stimuli, we compared the mRNA expression profiles of decidualized ESCs stimulated by cAMP or cAMP+MPA for 4 days, or by MPA or E2+MPA for 14 days.

2024-04-10 | GSE235151 | GEO

Genome wide RNA expression profile of endometrial stromal cells stimulated by cAMP, and cAMP under PGC-1a-knockdown

Project description:Genome wide RNA expression profile of endometrial stromal cells stimulated by cAMP, and cAMP under PGC-1a-knockdown

| PRJNA705712 | ENA

Genome-wide DNA methylation analysis revealed stable DNA methylation status during decidualization in human endometrial stromal cells.

Project description:BackgroundDuring decidualization in endometrial stromal cells (ESCs), expressions of a number of genes and epigenetic modifications of histones are altered. However, there is little information about whether DNA methylation, which is another epigenetic mechanism, also changes during decidualization. Here, we examined the genome-wide DNA methylation profiles in ESCs during decidualization and their associations with the changes of gene expressions and histone modifications.ResultsESCs were incubated with estradiol and medroxyprogesterone acetate for 14 days to induce decidualization. The genome-wide DNA methylation profiles were compared between the non-decidualized ESCs and the decidualized ESCs. Of 482,005 CpGs, only 23 CpGs (0.0048%) showed different DNA methylation statuses. The DNA methylation statuses of the differentially expressed genes and the regions with different histone modifications (H3K4 tri-methylation and H3K27 acetylation) were also compared between the ESCs. In the upregulated and downregulated genes in decidualized ESCs, DNA methylation statuses around the promoter region of the genes did not significantly differ between the ESCs. In the regions with different histone modification, DNA methylation statuses did not differ between the ESCs. The differentially expressed genes and the differential histone modification regions were hypomethylated.ConclusionsCulturing ESCs with estrogen/progesterone did not distort the physiological pattern of DNA methylation, although mRNA expression and histone modifications were dynamically altered. A genome-wide DNA methylation analysis revealed stable DNA methylation statuses during decidualization in human endometrial stromal cells. DNA hypomethylation is maintained for the variable changes of histone modifications and gene expression during decidualization.

| S-EPMC6489213 | biostudies-literature

Genome-wide analysis of histone modifications in human endometrial stromal cells.

Project description:Dramatic changes of gene expressions occur in human endometrial stromal cells (ESCs) during decidualization. The changes in gene expression are associated with changes of chromatin structure, which are regulated by histone modifications. Here we investigated genome-wide changes in histone modifications associated with decidualization in human ESCs using chromatin immunoprecipitation combined with next-generation sequencing. ESCs were incubated with estradiol and medroxyprogesterone acetate for 14 days to induce decidualization. The chromatin immunoprecipitation-sequence data showed that induction of decidualization increased H3K27ac and H3K4me3 signals in many genomic regions but decreased in only a few regions. Most of the H3K27ac-increased regions (80%) and half of the H3K4me3-increased regions were located in the distal promoter regions (more than 3 kb upstream or downstream of the transcription start site). RNA sequence showed that induction of decidualization up-regulated 881 genes, 223 of which had H3K27ac- or H3K4me3-increased regions in the proximal and distal promoter regions. Induction of decidualization increased the mRNA levels of these genes more than it increased the mRNA levels of genes without H3K27ac- or H3K4me3-increased regions. Pathway analysis revealed that up-regulated genes with the H3K27ac- or H3K4me3-increased regions were associated with the insulin signaling, which may be involved in glucose uptake that is necessary for ESCs to undergo decidualization. These results show that histone modification statuses on a genome-wide basis change in human ESCs during decidualization. The main changes of histone modifications are increases of H3K27ac and H3K4me3 in both the proximal and distal promoter regions, which are involved in the up-regulation of gene expression that occurs during decidualization.

| S-EPMC5414786 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data