Transcriptomics

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MNET sequencing facilitates identification of native elongating transcripts in control and Carm1 KO melanoma cells


ABSTRACT: We investigated whether RNA Pol II mediated transcription was altered in Carm1 KO compared to control KO tumor cells. We examined p-Ser2 CTD Pol II relative to total CTD Pol II using mammalian native elongating transcript sequencing (mNET-Seq) (Nojima et al., 2016). Inactivation of the Carm1 gene substantially increased the normalized read density of p-Ser2 CTD Pol II tags relative total Pol II tags. These data provide evidence for altered transcriptional regulation in Carm1 deficient cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE148905 | GEO | 2022/01/31

REPOSITORIES: GEO

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