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Investigation of colominic acid effects on immortalized Schwann cells

ABSTRACT: The purpose of the present study is to influence of CA coating on immortalized Schwann cells in vitro in comparison to standard culture conditions as well as to laminin as a positive standard. CA is a homopolymer of polysialic acid (PSA), which has – attached to the neuronal cell adhesion molecule (NCAM) – emerged as particulate attractive candidate for promoting morphological plasticity of axonal processes during axonal path finding and activity-dependent remodelling. For this purpose self-spotted microarrays with 352 neurospecific genes were hybridized with mRNA from immortalized Schwann cells cultivated on different coating conditions: In order to enable a comprehensive comparison to the established coating material laminin as well as to standard coating conditions (cultivation on the cell culture surface) a loop-design has been chosen, which is based on the dye-swap design and facilitates a direct comparison of the different states.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE14944 | GEO | 2009/02/25

SECONDARY ACCESSION(S): PRJNA111989

REPOSITORIES: GEO

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Investigation of colominic acid effects on immortalized Schwann cells

Project description:The purpose of the present study is to influence of CA coating on immortalized Schwann cells in vitro in comparison to standard culture conditions as well as to laminin as a positive standard. CA is a homopolymer of polysialic acid (PSA), which has â attached to the neuronal cell adhesion molecule (NCAM) â emerged as particulate attractive candidate for promoting morphological plasticity of axonal processes during axonal path finding and activity-dependent remodelling. For this purpose self-spotted microarrays with 352 neurospecific genes were hybridized with mRNA from immortalized Schwann cells cultivated on different coating conditions: In order to enable a comprehensive comparison to the established coating material laminin as well as to standard coating conditions (cultivation on the cell culture surface) a loop-design has been chosen, which is based on the dye-swap design and facilitates a direct comparison of the different states. Results from 3 microarrays are summarized in this study. The samples originate from different coatings. Microarrays were hybridized in a loop design with one common reference using a dye swap approach.

2010-06-23 | E-GEOD-14944 | biostudies-arrayexpress

Gene expression profiling of NCAM-deficient mouse brain

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Transcriptomic signature of treatment with recombinant laminin 511-E8 and acetazolamide in human H69 cholangiocytes

Project description:IgG4-related cholangitis (IRC) is the hepatobiliary manifestation of IgG4-related disease (IgG4-RD), a systemic, B-cell driven, fibroinflammatory disorder. To date, numerous autoantigens have been described including laminin 511-E8 and carbonic anhydrase (CA) enzymes (PMID 30089633, 15647194). Laminin 511-E8 is an extracellular matrix protein that has been shown to upregulate secretory components and aid in the cholangiocyte differentiation of induced pluripotent stem cells (PMID 27103433). Carbonic anhydrase enzymes catalyse the reversible hydration of carbon dioxide, thereby producing bicarbonate and a single proton. In humans there are 14 different CA isoforms with a confined subcellular distribution. All isoforms can be targeted by the pan-CA inhibitor acetazolamide, which is a registered drug for multiple indications. We aimed to investigate the role of IgG4-RD autoantigens laminin 511-E8 and CA enzymes in human cholangiocytes. Transcriptional profiling may provide insights into the underlying molecular mechanisms of these proteins on the cholangiocellular level. Thus, by RNA sequencing the transcriptomic signature of recombinant laminin 511-E8 and acetazolamide treatment was assessed in human H69 cholangiocytes.

2024-02-15 | GSE221746 | GEO

ACTL6a knockout peripheral nerve RNA seq at postnatal day 3

Project description:Cells need to integrate chemical and physical signals into transcriptional programs. In the peripheral nerves, axonal caliber selection by specialized Schwann cells, is critical for developmental myelination. However, only mechanisms through which Schwann cells sense chemical signals are well characterized. We identify ACtin-Like protein 6a (ACTL6a), a component of the SWI/SNF chromatin remodeling complex, as critical for axonal caliber recognition and developmental myelination. ACTL6a is expressed in the developing nerve and, when activated by contact with axons or nanofibers of specific caliber, it promotes the eviction of repressive histone marks thereby enhancing the transcriptional program of myelination. Mutant mice with Schwann cells lacking ACTL6a display aberrant recognition of axonal caliber, resulting in defective radial sorting and lower levels of transcripts regulating myelination of developing nerves. We suggest that developing peripheral nerves require an ACTL6a-dependent integration of physical and chemical signals in Schwann cells to release of repressive histone modifications and promote myelination.

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Gene expression profiling of NCAM-deficient mouse brain

Project description:Analysis of brains of mice lacking the neural cell adhesion molecule NCAM (Ncam-/-) in comparison to wild-type mice of same age and genetic background (Ncam+/+). NCAM-deficient mice exhibit deficits in long-term potentiation and spatial learning, as well as increased intermale aggression. We performed a microarray analysis of adult NCAM-deficient mouse brains to identify molecular targets which might underly the phenotype of NCAM-deficient mice. We analyzed the gene expression profiles in 2-3 months old (young adult) mouse brains, with both the Ncam-/- and the Ncam+/+ group consisting of three individuals each. Wild-type and NCAM-deficient animals had the same genetic background. RNA was prepared from total brain.

2009-12-01 | E-GEOD-16647 | biostudies-arrayexpress

Laminin binding/non-binding germ cells

Project description:Comparison of laminin binding and laminin non-binding germ cells Keywords: other

2004-03-16 | GSE829 | GEO

Sox10 Binding Analysis in the Central and Peripheral Nervous System

Project description:ChIP-seq of Sox10 in spinal cord and sciatic nerve was performed to determine shared and unique binding sites for Sox10 in oligodendrocytes and Schwann cells, respectively. Sox10 is required for both Schwann cell and oligodendrocyte development. In addition, differentiation of myelinating glia is dependent upon axonal signaling, so these studies were performed in vivo.

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Failures of nerve regeneration caused by aging or chronic denervation are rescued by restoring Schwann cell c-Jun

Project description:After nerve injury, myelin and Remak Schwann cells reprogram to repair cells specialized for regeneration. Normally providing strong regenerative support, these cells fail in aging animals, and during the chronic denervation that results from slow axon growth. This impairs axonal regeneration and causes a significant clinical problem. We find that aging and chronically denervated repair cells express reduced c-Jun protein and the regenerative support provided by these cells is also reduced. In both cases, genetically restoring Schwann cell c-Jun levels restores regeneration to that in controls. We identify potential gene candidates mediating this effect and implicate Shh in the control of Schwann cell c-Jun levels. These experiments reveal that a common mechanism, reduced c-Jun in repair cells, underlies two major reasons for regeneration failure in the PNS. They underscore the central importance of Schwann cell c-Jun as a regulator of nerve repair, and point to molecular pathways that can be manipulated for improving the clinical outcome of nerve injuries.

2020-10-14 | E-MTAB-9640 | biostudies-arrayexpress

IL-22 impedes the proliferation of Schwann cells and influence nerve regeneration

Project description:Schwann cells (SCs) proliferation is crucial for axonal guidance and nerve regeneration following nerve injury. This study aims to investigate the in vitro effects of interleukin-22 (IL-22) on SCs and the corresponding mechanism.

2018-06-15 | GSE69866 | GEO

Timecourse expression data from sciatic nerve distal stump of C57BL/6 and C57BL/6 OlaHsd-Wlds mice.

Project description:Wallerian degeneration (WD) involves the fragmentation of axonal segments disconnected from their cell bodies, segmentation of the myelin sheath, and removal of debris by Schwann cells and immune cells. The removal and downregulation of myelin-associated inhibitors of axonal regeneration and synthesis of growth factors by these two cell types are critical responses to successful nerve repair. Here, we analyzed the transcriptome of the sciatic nerve of mice carrying the Wallerian degeneration slow (WldS) mutant gene, a gene that confers axonal protection in the distal stump after injury, therefore causing significant delays in WD, neuroinflammation, and axonal regeneration.

2011-06-01 | GSE22291 | GEO

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