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THE FUSED LEAVES1-ADHERENT1 REGULATORY MODULE IS REQUIRED FOR MAIZE CUTICLE DEVELOPMENT AND ORGAN SEPARATION

ABSTRACT: RNA-seq was performed on 3-4mm WT and fdl1 mutant maize coleoptile tips

ORGANISM(S): Zea mays

PROVIDER: GSE149577 | GEO | 2020/08/06

REPOSITORIES: GEO

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The FUSED LEAVES1-ADHERENT1 regulatory module is required for maize cuticle development and organ separation.

Project description:All aerial epidermal cells in land plants are covered by the cuticle, an extracellular hydrophobic layer that provides protection against abiotic and biotic stresses and prevents organ fusion during development. Genetic and morphological analysis of the classic maize adherent1 (ad1) mutant was combined with genome-wide binding analysis of the maize MYB transcription factor FUSED LEAVES1 (FDL1), coupled with transcriptional profiling of fdl1 mutants. We show that AD1 encodes an epidermally-expressed 3-KETOACYL-CoA SYNTHASE (KCS) belonging to a functionally uncharacterized clade of KCS enzymes involved in cuticular wax biosynthesis. Wax analysis in ad1 mutants indicates that AD1 functions in the formation of very-long-chain wax components. We demonstrate that FDL1 directly binds to CCAACC core motifs present in AD1 regulatory regions to activate its expression. Over 2000 additional target genes of FDL1, including many involved in cuticle formation, drought response and cell wall organization, were also identified. Our results identify a regulatory module of cuticle biosynthesis in maize that is conserved across monocots and eudicots, and highlight previously undescribed factors in lipid metabolism, transport and signaling that coordinate organ development and cuticle formation.

| S-EPMC7754373 | biostudies-literature

THE FUSED LEAVES1-ADHERENT1 REGULATORY MODULE IS REQUIRED FOR MAIZE CUTICLE DEVELOPMENT AND ORGAN SEPARATION

Project description:THE FUSED LEAVES1-ADHERENT1 REGULATORY MODULE IS REQUIRED FOR MAIZE CUTICLE DEVELOPMENT AND ORGAN SEPARATION

| PRJNA629302 | ENA

needle1 (ndl1) encodes a mitochondria localized ATPase required for thermotolerant maize growth [field]

Project description:RNA-seq was performed on 2-4mm WT and ndl1 mutant maize tassels

2019-08-06 | GSE129680 | GEO

needle1 (ndl1) encodes a mitochondria localized ATPase required for thermotolerant maize growth [greenhouse]

Project description:RNA-seq was performed on 2-4mm WT and Bif1;Bif4 double heterozygous mutant maize tassels grown in a greenhouse

2019-08-06 | GSE129683 | GEO

ADHERENT1 IS A 3-KETOACYL CoA SYNTHASE REQUIRED FOR MAIZE CUTICLE DEVELOPMENT AND ORGAN SEPARATION

Project description:DAP-seq was used to generate genome-wide DNA:TF interaction maps for ZmMYB94/FDL1

2020-06-01 | GSE142847 | GEO

FAIRE-seq of the first leaf of two maize lines of contrasting cold-sensitivity.

Project description:High-throughput sequencing of genomic regions isolated using FAIRE (Formaldehyde-assisted isolation of regulatory elements) from two maize lines of contrasting cold-sensitivity, S68911 (tolerant) and B73 (sensitive) grown in cold and control conditions. Three growth stages were examined: coleoptile (VE), seedling with the tip of the second leaf visible (called here “VE/V1 stage”), first leaf fully developed (V1, ligular region present). Results suggest both efficient metabolism and active defense mechanisms as a basis of S68911 maize cold-tolerance.

2020-10-19 | E-MTAB-8401 | biostudies-arrayexpress

ADHERENT1 IS A 3-KETOACYL CoA SYNTHASE REQUIRED FOR MAIZE CUTICLE DEVELOPMENT AND ORGAN SEPARATION

Project description:ADHERENT1 IS A 3-KETOACYL CoA SYNTHASE REQUIRED FOR MAIZE CUTICLE DEVELOPMENT AND ORGAN SEPARATION

| PRJNA598606 | ENA

Single cell RNA sequencing of maize root

Project description:The root system is fundamental for maize growth and yield. The primary root system is the most important structure of maize seedlings and is the first organ that emerges at germination, providing water and nutrients for the growing seedlings. However, it is difficult to characterize them at single cell level, due to their complex and heterogeneous cell types. In this study, we profiled the transcriptomes of more than 7000 cells derived from maize root tips of seedlings grown on media with (nitrate+) or without nitrate (nitrate-).

2021-09-03 | GSE183171 | GEO

The maize fused leaves1 (fdl1) gene controls organ separation in the embryo and seedling shoot and promotes coleoptile opening.

Project description:The fdl1-1 mutation, caused by an Enhancer/Suppressor mutator (En/Spm) element insertion located in the third exon of the gene, identifies a novel gene encoding ZmMYB94, a transcription factor of the R2R3-MYB subfamily. The fdl1 gene was isolated through co-segregation analysis, whereas proof of gene identity was obtained using an RNAi strategy that conferred less severe, but clearly recognizable specific mutant traits on seedlings. Fdl1 is involved in the regulation of cuticle deposition in young seedlings as well as in the establishment of a regular pattern of epicuticular wax deposition on the epidermis of young leaves. Lack of Fdl1 action also correlates with developmental defects, such as delayed germination and seedling growth, abnormal coleoptile opening and presence of curly leaves showing areas of fusion between the coleoptile and the first leaf or between the first and the second leaf. The expression profile of ZmMYB94 mRNA-determined by quantitative RT-PCR-overlaps the pattern of mutant phenotypic expression and is confined to a narrow developmental window. High expression was observed in the embryo, in the seedling coleoptile and in the first two leaves, whereas RNA level, as well as phenotypic defects, decreases at the third leaf stage. Interestingly several of the Arabidopsis MYB genes most closely related to ZmMYB94 are also involved in the activation of cuticular wax biosynthesis, suggesting deep conservation of regulatory processes related to cuticular wax deposition between monocots and dicots.

| S-EPMC4566974 | biostudies-literature

MALDI-MS imaging of 13C labeled maize root tips

Project description:MADLI MS imaging dataset of developing maize root tips grown in 13C or unlabeled glucose. Manuscript is being submitted and the citation will be made once it is published.

2023-01-06 | MSV000091017 | MassIVE

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