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Understanding the functional role of cyanidin-3-O-glucoside in mouse osteoblastic cell line MC3T3-E1 by RNA-seq analysis

ABSTRACT: Cyanidin-3-O-glucoside (C3G) is one of the most widely distributed anthocyanins and has the effect of reducing the risks of osteoporosis. However, the molecular mechanism by which C3G promotes bone formation remains incompletely understood. Our aim was to investigate the effect of C3G on gene expression profiles, during the proliferation and differentiation in MC3T3-E1, a kind of osteoblast cell line from mice, by RNA sequencing. The results identified 34 up-regulated and 17 down-regulated genes. We further performed Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes analyses (KEGG), and other bioinformatics methods to do further analysis of differentially expressed genes (DEGs). Analysis of sequencing data showed that these genes were highly enriched in functions related to Lysosomal and Glycolipid biosynthetic. Lastly, we used RT-qPCR to validate the differentially expressed mRNAs. The results showed that C3G increased the expression levels of Ly6a (Lymphocyte antigen 6 family member A), Atp6v0c (ATPase H+ transporting v0 subunit c), and decreased the expression levels of Cx3cl1 (C-X3-C motif chemokine ligand 1). This study suggested that C3G activated osteoblast via Glycosylphosphatidylinositol(GPI)-anchor biosynthesis and the Cx3cl1/Cx3cr1 signaling pathway.

ORGANISM(S): Mus musculus

PROVIDER: GSE149731 | GEO | 2022/10/19

REPOSITORIES: GEO

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