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M. sedula transcriptional response under heterotrophic, autotrophic, and mixotrophic growth conditions

ABSTRACT: To study mixotrophy, it is desirable to have an organism capable of growth in the presence and absence of both organic and inorganic carbon sources, as well as organic and inorganic energy sources. Metallosphaera sedula is an extremely thermoacidophilic archaeon which has been shown to grow in the presence of inorganic carbon and energy source supplements (autotrophy), organic carbon and energy source supplements (heterotrophy), and in the presence of organic carbon and inorganic energy source supplements. The recent elucidation of M. sedula’s inorganic carbon fixation cycle and its genome sequence further facilitate its use in mixotrophic studies. In this study, we grow M. sedula heterotrophically in the presence of organic carbon and energy sources (0.1% tryptone), autotrophically in the presence of inorganic carbon and energy sources (H2 + CO2), and “mixotrophically” in the presence of both organic and inorganic carbon and energy sources (0.1% tryptone + H2 + CO2 ) to characterize the nature of mixotrophy exhibited.

ORGANISM(S): Metallosphaera sedula

PROVIDER: GSE14978 | GEO | 2009/11/01

SECONDARY ACCESSION(S): PRJNA111881

REPOSITORIES: GEO

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M. sedula transcriptional response under heterotrophic, autotrophic, and mixotrophic growth conditions

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