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Genome-wide profiling of RNA Polymerase II, MITF and DDX21 occupancy in human A375 cells stably expressing either empty vector or HA-tagged PRL3.

ABSTRACT: To determine the role of PRL3 in gene regulation, empty vector or HA-tagged PRL3 was stably expressed in cultured human melanoma A375 cells. ChIP-seq was used to determine the impact of increased PRL3 expression on the genome-wide distribution of RNA Polymerase II (Total/Ser5-Phosphorylated/Ser2-Phosphorylated), MITF and DDX21. In combination with transcriptional analysis (4sU-seq; GEO accession - GSE127855), this study demonstrated that PRL3 overexpression leads to reduced RNA polymerase processivity at a subset of so called 'restricted' genes which are enriched for endolysosomal functions.

ORGANISM(S): Homo sapiens

PROVIDER: GSE149929 | GEO | 2020/07/20

REPOSITORIES: GEO

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RNA helicase DDX21 coordinates transcription and noncoding RNA processing of the ribosomal pathway

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