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Transcriptomic and epigenetic analysis of neonatal supporting cells during Notch inhibition-induced transdifferentiation

ABSTRACT: Latent regeneration potential has been found in neonatal supporting cells in the organ of Corti in mouse. Upon Notch inhibition by DAPT, postnatal day 1 (P1) supporting cells transdifferentiate into hair cells. To profile the transcriptomic and epigenetic changes in supporting cells during transdifferentiation, we FACS purified supporting cells from DAPT treated cochleae for RNAseq, ATACseq and H3K27ac CUT&RUN. We found that hair cell genes were up-regulated in supporting cells after DAPT treatment and that hair cell gene induction was accompanied by epigentic activation of hair cell gene cis-regulatory elements through chromatin accessibility increase and H3K27ac accumulation, suggesting the regulatory roles of epigenetic activation of hair cell gene elements for hair cell gene expression induction.

ORGANISM(S): Mus musculus

PROVIDER: GSE150002 | GEO | 2021/07/09

REPOSITORIES: GEO

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Epigenetic analysis of perinatal maturation of supporting cells in the organ of Corti

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