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SASP signature of beta-cells includes pro-inflammatory and stress response proteins

ABSTRACT: To define the senescence-associated secretory phenotype (SASP) of beta-cells, we used conditioned media (CM) generated from bleomycin-treated MIN6 cells and from senescent (beta-Gal-positive) primary beta-cells. In order to culture senescent beta-cells, we isolated islet, FACS-sorted them into beta-Gal-positive and negative populations, excluding immune cells through negative selection of CD45-positive and CD11beta-positive cells. For both the MIN6 and primary beta-cell models, we cultured cells in serum-free media to generate CM for proteomic analysis using the aptamer-based SomaScan platform.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE150285 | GEO | 2021/04/06

REPOSITORIES: GEO

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