Genomics

Dataset Information

0

Small RNA libraries from epidermal stem cell (ESC) and fibroblast (Fb) derived exosomes


ABSTRACT: We employed high-throughput sequencing to profile the non-coding RNA content of primary ESC and Fb derived exosomes. All 6 libraries were sequenced to a comparable depth (ESC1: 12.3M, ESC2: 14M, ESC3: 12.8M, FB1: 14.7M, FB2: 13.7M, FB3: 12.3M) and exhibited a very low proportion of adapter-only reads or fragments shorter than 17bp (4.4 ± 0.5% ), showing efficient fragment incorporation during library preparation. All libraries had high small RNA content (>50% successfully aligned and assigned to small RNAs). ESC libraries had significantly higher small RNA content (ESC: 84.5 ± 0.005% vs FB: 60.5 ± 0.05%, p<0.05, two-sided Welch’s t-test). ESC samples show remarkably higher proportions of miRNAs (~9-fold higher, p=3.6*10-5 ) and snRNAs (~12-fold higher, p=0.002) compared to Fb. Fibroblasts exhibited higher RPM of reads mapping on lncRNAs (4-fold, p=0.052)

ORGANISM(S): Mus musculus

PROVIDER: GSE150765 | GEO | 2020/10/29

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

| PRJNA633535 | ENA
| PRJNA266747 | ENA
| PRJNA178046 | ENA
| PRJNA187096 | ENA
| PRJNA239509 | ENA
| PRJNA309719 | ENA
| PRJNA293114 | ENA
| PRJNA293116 | ENA
| PRJNA293113 | ENA
| PRJNA505563 | ENA