Transcriptomics

Dataset Information

0

Monocyte Subsets with High Osteoclastogenic Potential and Their Epigenetic Regulation Orchestrated by IRF8 [RNA-Seq]


ABSTRACT: Currently, it is unclear if all monocyte subsets exhibit osteoclastogenic potential. Furthermore, the role of lineage determining TFs in regulating OC differentiation on a genome-wide scale remains poorly understood. In this study, we utilized a novel Irf8 conditional knockout (Irf8 cKO) mouse model to characterize the importance of IRF8 in OC progenitor development and epigenetic regulation of OC differentiation. We performed RNA-seq on Irf8 gWT, gKO, cWT and cKO BMMs, preosteoclasts (PreOCs), and OCs. Cells were analyzed prior to (BMMs) and 3 days (PreOCs) and 6 days (OCs) after RANKL stimulation. Here we show that greater a number of genes are significantly upregulated in Irf8 cKO OCs when compared to WT and Irf8 gKO OCs. Altogether, our data shows that Irf8 cKO mice provide more precise/reliable OC-specific transcriptomic results when compared to Irf8 gKO mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE151388 | GEO | 2020/09/11

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2020-09-11 | GSE151480 | GEO
2020-09-19 | GSE151481 | GEO
| PRJNA635933 | ENA
| PRJNA635936 | ENA
| PRJNA635657 | ENA
| S-EPMC8168257 | biostudies-literature
2019-06-01 | GSE115497 | GEO
| PRJNA635935 | ENA
2022-04-08 | PXD031791 | Pride
2008-06-15 | E-GEOD-676 | biostudies-arrayexpress