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Synthetic immunomodulation with a CRISPR super-repressor in vivo

ABSTRACT: In this study we introduce an enhanced CRISPR-based transcriptional repressor to reprogram immune homeostasis in vivo. By a dual fusion of heterochromatin protein 1 (HP1a) and Krüppel associated box (KRAB) to MS2 coat protein and recruitment to a nuclease competent CRISPR complex, we demonstrate transcriptional repression of the Myeloid differentiation primary response 88 (Myd88) gene in vivo. We report that this strategy can effectively modulate host immune response against adeno-associated virus-mediated gene therapy and influence the course of septicemia.

ORGANISM(S): Mus musculus

PROVIDER: GSE152412 | GEO | 2020/07/07

REPOSITORIES: GEO

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