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Control of a programmed cell death pathway in Pseudomonas aeruginosa by a ppGpp-responsive antiterminator

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Pseudomonas aeruginosa PAO1

PROVIDER: GSE152485 | GEO | 2021/01/19

REPOSITORIES: GEO

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Control of a programmed cell death pathway in Pseudomonas aeruginosa by a ppGpp-responsive antiterminator [ChIP-Seq]

Project description:In the opportunistic pathogen Pseudomonas aeruginosa the alp system encodes a programmed cell death (PCD) pathway that is switched on in a subset of cells in response to DNA damage. Here we show that the central regulator of this pathway, AlpA, exerts its effects by acting as an antiterminator rather than a transcription activator. In particular, we present evidence that AlpA positively regulates the alpBCDE cell lysis genes, as well as genes in a second newly identified operon, by recognizing specific DNA sites within the promoter, then binding RNA polymerase directly and allowing it to bypass intrinsic terminators positioned downstream. We further show that the activity of AlpA is potentiated by the alarmone ppGpp, which is produced by the cell in response to stress. Through its responsiveness to ppGpp, AlpA can integrate environmental cues into the decision to execute a PCD pathway that is linked to the virulence of the organism.

2021-01-19 | GSE152481 | GEO

Control of a programmed cell death pathway in Pseudomonas aeruginosa by a ppGpp-responsive antiterminator [RNAtag-seq]

2021-01-19 | GSE152480 | GEO

Control of a programmed cell death pathway in Pseudomonas aeruginosa by a ppGpp-responsive antiterminator [RNA-Seq]

2021-01-19 | GSE152484 | GEO

Control of a programmed cell death pathway in Pseudomonas aeruginosa by an antiterminator.

Project description:In Pseudomonas aeruginosa the alp system encodes a programmed cell death pathway that is switched on in a subset of cells in response to DNA damage and is linked to the virulence of the organism. Here we show that the central regulator of this pathway, AlpA, exerts its effects by acting as an antiterminator rather than a transcription activator. In particular, we present evidence that AlpA positively regulates the alpBCDE cell lysis genes, as well as genes in a second newly identified target locus, by recognizing specific DNA sites within the promoter, then binding RNA polymerase directly and allowing it to bypass intrinsic terminators positioned downstream. AlpA thus functions in a mechanistically unusual manner to control the expression of virulence genes in this opportunistic pathogen.

| S-EPMC7969949 | biostudies-literature

Control of a programmed cell death pathway in Pseudomonas aeruginosa by a ppGpp-responsive antiterminator

Project description:Control of a programmed cell death pathway in Pseudomonas aeruginosa by a ppGpp-responsive antiterminator

| PRJNA639519 | ENA

Control of a programmed cell death pathway in Pseudomonas aeruginosa by a ppGpp-responsive antiterminator [RNAtag-seq]

Project description:Control of a programmed cell death pathway in Pseudomonas aeruginosa by a ppGpp-responsive antiterminator [RNAtag-seq]

| PRJNA639521 | ENA

Control of a programmed cell death pathway in Pseudomonas aeruginosa by a ppGpp-responsive antiterminator [ChIP-Seq]

Project description:Control of a programmed cell death pathway in Pseudomonas aeruginosa by a ppGpp-responsive antiterminator [ChIP-Seq]

| PRJNA639522 | ENA

Control of a programmed cell death pathway in Pseudomonas aeruginosa by a ppGpp-responsive antiterminator [RNA-Seq]

Project description:Control of a programmed cell death pathway in Pseudomonas aeruginosa by a ppGpp-responsive antiterminator [RNA-Seq]

| PRJNA639523 | ENA

Pseudomonas aeruginosa PA14 MvfR-dependent QS regulatory pathway

Project description:This SuperSeries is composed of the SubSeries listed below.

2009-07-17 | GSE17147 | GEO

phoU inactivation in Pseudomonas aeruginosa enhances accumulation of ppGpp and polyphosphate.

Project description:Inorganic polyphosphate (polyP) is a linear polymer composed of several molecules of orthophosphate (Pi) linked by energy-rich phosphoanhydride bonds. In Pseudomonas aeruginosa, Pi is taken up by the ABC transporter Pst, encoded by an operon consisting of five genes. The first four genes encode proteins involved in the transport of Pi and the last gene of the operon, phoU, codes for a protein which exact function is unknown. We show here that the inactivation of phoU in P. aeruginosa enhanced Pi removal from the medium and polyP accumulation. The phoU mutant also accumulated high levels of the alarmone guanosine tetraphosphate (ppGpp), which in turn increased the buildup of polyP. In addition, phoU inactivation had several pleiotropic effects, such as reduced growth rate and yield and increased sensitivity to antibiotics and stresses. However, biofilm formation was not affected by the phoU mutation.

| S-EPMC4393453 | biostudies-literature

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