Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Heritable enhanced growth vigor through grafting is associated with the RdDM pathway in plants [Tomato_graft_RNAseq]

ABSTRACT: RNA sequencing of progenies of Rutgers CV. Wild type grafted on Rutgers transgenic MSH1-RNAi ; Rutgers CV. Wild type grafted on Rutgers Wild type

ORGANISM(S): Solanum lycopersicum

PROVIDER: GSE152569 | GEO | 2020/09/20

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Heritable enhanced growth vigor through grafting is associated with the RdDM pathway in plants [tomato_graft_methylome]

Project description:Bisulfite (methylome) sequencing of progenies of Rutgers CV. Wild type grafted on Rutgers MSH1-RNAi line; Rutgers CV. Wild type grafted on Rutgers Wild type

2020-09-20 | GSE152568 | GEO

MSH1-induced heritable enhanced growth vigor through grafting is associated with the RdDM pathway in plants.

Project description:Plants transmit signals long distances, as evidenced in grafting experiments that create distinct rootstock-scion junctions. Noncoding small RNA is a signaling molecule that is graft transmissible, participating in RNA-directed DNA methylation; but the meiotic transmissibility of graft-mediated epigenetic changes remains unclear. Here, we exploit the MSH1 system in Arabidopsis and tomato to introduce rootstock epigenetic variation to grafting experiments. Introducing mutations dcl2, dcl3 and dcl4 to the msh1 rootstock disrupts siRNA production and reveals RdDM targets of methylation repatterning. Progeny from grafting experiments show enhanced growth vigor relative to controls. This heritable enhancement-through-grafting phenotype is RdDM-dependent, involving 1380 differentially methylated genes, many within auxin-related gene pathways. Growth vigor is associated with robust root growth of msh1 graft progeny, a phenotype associated with auxin transport based on inhibitor assays. Large-scale field experiments show msh1 grafting effects on tomato plant performance, heritable over five generations, demonstrating the agricultural potential of epigenetic variation.

| S-EPMC7582163 | biostudies-literature

Heritable enhanced growth vigor through grafting is associated with the RdDM pathway in plants

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-09-20 | GSE152570 | GEO

RNA sequencing of progenies of Col-0 grafted on msh1; Col-0 grafted on dcl2,3,4,msh1 quadruple mutant in Arabidopsis [msh1_graft_root_RNA-seq]

Project description:Col-0 floral stem was grafted on the msh1 mutant (Col-0/msh1) and on the dcl2,3,4,msh1 quadruple mutant (Col-0/dcl2,3,4,msh1). Seeds were collected from the grafted Col-0 scion after grafts were established. Seed coming from the graft then were grown on 0.5M MS growth medium and root tissue was collected after 12 days and used for the RNA sequencing.

2020-09-20 | GSE152566 | GEO

Total RNA sequencing of progenies of Col-0 grafted on msh1; Col-0 grafted on dcl2,3,4,msh1; Col-0 grafted on Col-0 in Arabidopsis [msh1_graft_leaf_RNA-seq]

2020-09-20 | GSE152565 | GEO

Small RNA sequencing of progenies of Col-0 grafted on msh1; Col-0 grafted on dcl2,3,4,msh1; msh1 mutant; dcl2,3,4,msh1 quadruple mutant in Arabidopsis [msh1_graft_sRNA-seq]

Project description:Col-0 floral stem was grafted on the msh1 mutant (Col-0/msh1); on the dcl2,3,4,msh1 quadruple mutant (Col-0/dcl2,3,4,msh1). Seeds were collected from the grafted Col-0 scion after grafts were established. Seed coming from the graft then were grown on the peat mix, leaf tissue was collected at the bolting and used for the small RNA sequencing. Tissue from the msh1 mutant and dcl2,3,4,msh1 quadruple mutants used as rootstocks was similarly collected at the bolting stage and used for the small RNA sequencing.

2020-09-20 | GSE152567 | GEO

Bisulfite (methylome) sequencing of progenies of Col-0 grafted on msh1; Col-0 grafted on dcl2,3,4,msh1; Col-0 grafted on Col-0; msh1 mutant; dcl2,3,4,msh1 quadruple mutant in Arabidopsis [msh1_graft_BS-seq]

Project description:Col-0 floral stem was grafted on the msh1 mutant (Col-0/msh1); on the dcl2,3,4,msh1 quadruple mutant (Col-0/dcl2,3,4,msh1); on Col-0 (Col-0/Col-0). Seeds were collected from the grafted Col-0 scion after grafts were established. Seed coming from the graft then were grown on the peat mix, leaf tissue was collected at the bolting and used for the bisulfite sequencing (methylome). Tissue from the msh1 mutant and dcl2,3,4,msh1 quadruple mutants used as rootstocks was similarly collected at the bolting stage and used for the bisulfite sequencing.

2020-09-20 | GSE152564 | GEO

Heritable enhanced growth vigor through grafting is associated with the RdDM pathway in plants

Project description:Heritable enhanced growth vigor through grafting is associated with the RdDM pathway in plants

| PRJNA639725 | ENA

Heritable enhanced growth vigor through grafting is associated with the RdDM pathway in plants [tomato_graft_methylome]

Project description:Heritable enhanced growth vigor through grafting is associated with the RdDM pathway in plants [tomato_graft_methylome]

| PRJNA639735 | ENA

Heritable enhanced growth vigor through grafting is associated with the RdDM pathway in plants [Tomato_graft_RNAseq]

Project description:Heritable enhanced growth vigor through grafting is associated with the RdDM pathway in plants [Tomato_graft_RNAseq]

| PRJNA639736 | ENA