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High-resolution quantitative profiling of tRNA abundance and modification status in eukaryotes by mim-tRNAseq

ABSTRACT: Measurements of cellular tRNA abundance are hampered by pervasive blocks to cDNA synthesis at modified nucleosides and the extensive similarity among tRNA genes. We overcome these limitations with modification-induced misincorporation tRNA sequencing (mim-tRNAseq), which combines a workflow for full-length cDNA library construction from mature tRNA with a simple-to-use computational analysis toolkit. Our method accurately captures tRNA abundance and modification status in multiple eukaryotes and is applicable to any organism with a known genome.

ORGANISM(S): Schizosaccharomyces pombe Homo sapiens Saccharomyces cerevisiae Drosophila melanogaster

PROVIDER: GSE152621 | GEO | 2021/02/12

REPOSITORIES: GEO

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