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Fate and Functional Roles of Prominin 1+ Cells in Liver Injury and Cancer

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE152748 | GEO | 2020/12/17

REPOSITORIES: GEO

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Fate and functional roles of Prominin 1<sup>+</sup> cells in liver injury and cancer.

Project description:Prominin 1 (PROM1) is one of a few clinically relevant progenitor markers in human alcoholic hepatitis (AH) and hepatocellular carcinoma (HCC), and mouse liver tumor initiating stem cell-like cells (TICs). However, the origin, fate and functions of PROM1+ cells in AH and HCC are unknown. Here we show by genetic lineage tracing that PROM1+ cells are derived in part from hepatocytes in AH and become tumor cells in mice with diethyl nitrosamine (DEN)-initiated, Western alcohol diet-promoted liver tumorigenesis. Our RNA sequencing analysis of mouse PROM1+ cells, reveals transcriptomic landscapes indicative of their identities as ductular reaction progenitors (DRPs) and TICs. Indeed, single-cell RNA sequencing reveals two subpopulations of Prom1+ Afp- DRPs and Prom1+ Afp+ TICs in the DEN-WAD model. Integrated bioinformatic analysis identifies Discodin Domain Receptor 1 (DDR1) as a uniquely upregulated and patient-relevant gene in PROM1+ cells in AH and HCC. Translational relevance of DDR1 is supported by its marked elevation in HCC which is inversely associated with patient survival. Further, knockdown of Ddr1 suppresses the growth of TICs and TIC-derived tumor growth in mice. These results suggest the importance of PROM1+ cells in the evolution of liver cancer and DDR1 as a potential driver of this process.

| S-EPMC7656457 | biostudies-literature

Fate and Functional Roles of Prominin 1+ Cells in Liver Injury and Cancer [Quant-seq]

Project description:Purpose: The goal of the experiment is to obtain RNAseq profiles of Prom1+ Ductular Reactive Progenitors (DRPs) from mouse model of severe alcoholic liver injury Method: mRNA-seq analysis was performed in FACS sorted Prom1+CD49f+CD45-, Prom1-CD49f+CD45-, Prom1+CD49f+CD45+ cells and presorted CD45- liver cells. Conclusion: We obtained liver specific Prom1+ genes from analysis.

2020-12-17 | GSE152718 | GEO

Fate and Functional Roles of Prominin 1+ Cells in Liver Injury and Cancer [scRNA-seq]

Project description:Purpose: The goal of the study is to understand gene expression profiles of Prom1+ cells at single cell level in mouse liver cancer. Method: DEN-WAD liver from Col1a1Cre;Rosa26mTmG was perfused with collagenase. GFP+UV- cells were FACSsorted. Single cell library was constructed with 10X single gene expression v3 with 10X chromium technology. Conclusion: We identified Prom1+Afp+, Prom1+Afp-, Prom1-Afp+ gene expression profiles. These gene expression profiles revealed the markers previously observed for liver tumor intiating cells and ductular cells. In addition, they revealed novel specific markers.

2020-12-17 | GSE152707 | GEO

Fate and Functional Roles of Prominin 1+ Cells in Liver Injury and Cancer

Project description:Fate and Functional Roles of Prominin 1+ Cells in Liver Injury and Cancer

| PRJNA640317 | ENA

Drug-induced liver injury

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-01-22 | E-GEOD-54257 | biostudies-arrayexpress

Fate and Functional Roles of Prominin 1+ Cells in Liver Injury and Cancer [Quant-seq]

Project description:Fate and Functional Roles of Prominin 1+ Cells in Liver Injury and Cancer [Quant-seq]

| PRJNA640336 | ENA

Fate and Functional Roles of Prominin 1+ Cells in Liver Injury and Cancer [scRNA-seq]

Project description:Fate and Functional Roles of Prominin 1+ Cells in Liver Injury and Cancer [scRNA-seq]

| PRJNA640163 | ENA

Hippo Pathway Activity Influences Liver Cell Fate

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-06-06 | E-GEOD-55560 | biostudies-arrayexpress

Drug-induced liver injury

Project description:This SuperSeries is composed of the SubSeries listed below.

2014-01-22 | GSE54257 | GEO

Comprehensive Identification of Long Non-coding RNAs in Purified Cell Types from the Brain Reveals Functional LncRNA in OPC Fate Determination

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-11-04 | E-GEOD-74648 | biostudies-arrayexpress

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