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Single cell transcriptomes of brain metastases and micrometastases derived from triple negative human breast cancer cell line in mouse model.

ABSTRACT: In order to gainan understanding of potential mechanisms by which dystroglycan could effect DTC quiescence signaling in brain, we took an unbiased approach. We inoculated a series of mice with a tdTomato-positive, brain metastatic variant of T4-2 cells, and examined mice after 6-weeks in order to identify those with distinct brain metastases. Metastatic lesions were surgically dissected, digested and flow sorted to purify tdTomato+ cells (“Metastases”). In parallel, the remaining uninvolved brain was digested, and residual tdTomato+tumour cells were purified from this digest via flow sorting (“Micromets”). These two populations were indexed, and the libraries were prepared following standard 10X Genomics protocols.

ORGANISM(S): Homo sapiens

PROVIDER: GSE152818 | GEO | 2021/10/05

REPOSITORIES: GEO

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