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A systematic survey of PRMT interactomes reveals key roles of arginine methylation in the global control of RNA splicing and translation [Ribo-Seq]

ABSTRACT: We used Ribo-seq to determine how PRMT inhibition affects translation of different mRNAs, This analysis can generate a “snapshot” of all mRNAs that are occupied by active ribosomes (i.e., undergoing active translation) in a cell at a particular condition.

ORGANISM(S): Homo sapiens

PROVIDER: GSE153142 | GEO | 2022/06/30

REPOSITORIES: GEO

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A systematic survey of PRMT interactomes reveals key roles of arginine methylation in the global control of RNA splicing and translation [Poly-Seq]

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A systematic survey of PRMT interactomes reveals key roles of arginine methylation in the global control of RNA splicing and translation [RNA-seq]

Project description:Since many proteins involved in splicing regulation were identified as PRMT substrates, we examined the impact of PRMT inhibition on alternative RNA splicing. We achieved effective gene silence with shRNAs in six different PRMTs and examined their effect on splicing using RNA-seq.

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A systematic survey of PRMT interactomes reveals key roles of arginine methylation in the global control of RNA splicing and translation [Ribo-Seq]

Project description:A systematic survey of PRMT interactomes reveals key roles of arginine methylation in the global control of RNA splicing and translation [Ribo-Seq]

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Histone arginine methylation by Prmt controls lung branching morphogenesis through transcriptional repression of Bmp

Project description:We report that a protein arginine methyltransferase Prmt and symmetric dimethylation at histone H arginine (HRsme) directly associates with chromatin of Bmp to suppress its transcription. Inactivation of Prmt in the lung epithelium results in halted branching morphogenesis, altered P-D airway patterning and neonatal lethality.

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A systematic survey of PRMT interactomes reveals key roles of arginine methylation in the global control of RNA splicing and translation

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A systematic survey of PRMT interactomes reveals key roles of arginine methylation in the global control of RNA splicing and translation [RNA-seq]

Project description:A systematic survey of PRMT interactomes reveals key roles of arginine methylation in the global control of RNA splicing and translation [RNA-seq]

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A systematic survey of PRMT interactomes reveals key roles of arginine methylation in the global control of RNA splicing and translation [Poly-Seq]

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Leishmania braziliensis Protein Arginine Methyltransferases interactomes

Project description:We functionally characterized the five predicted PRMTs in Leishmania braziliensis by gene knockout and endogenous protein HA tagging using CRISPR/Cas9 gene editing. We report that R-methylation profiles vary among Leishmania species and across L. braziliensis lifecycle stages, with the peak PRMT expression occurring in promastigotes. A list of PRMT-interacting proteins was obtained in a single coimmunoprecipitation assay using HA-tagged PRMTs, suggesting a network of putative targets of PRMTs and cooperation between the R-methylation writers. Knockout of each L. braziliensis PRMT led to significant changes in global arginine methylation patterns without affecting cell viability. Deletion of either PRMT1 or PRMT3 disrupted most type I PRMT activity, resulting in a global increase in monomethyl arginine levels. Using anti-MMA antibodies, we performed an IP experiment to identify MMA proteins in the parental line, single PRMT1 knockout, PRMT1/PRMT7 double knockout and PRMT1-Addback parasites. The results indicate that R-methylation is modulated across lifecycle stages in L. braziliensis and show possible functional overlap and cooperation among the different PRMTs in targeting proteins. Overall, our data suggest important regulatory roles of these proteins throughout the L. braziliensis life cycle, showing that arginine methylation is important for parasite-host cell interactions. Linked publication: https://doi.org/10.1101/2021.09.22.461376.

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A systematic survey of PRMT interactomes reveals key roles of arginine methylation in the global control of RNA splicing and translation

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-06-30 | GSE153144 | GEO

Proteomics profiling of arginine methylation mediated by PRMT family reveals a critical role of arginine methylation in RNA splicing and cancer cell growth

Project description:Protein arginine methylation, catalyzed by the protein arginine methyltransferase (PRMT) family, is recognized as a widespread post-translational modification (PTM) with implications in a plethora of biological processes in eukaryotes. PRMT proteins were classified into three types, type I, II and III, according to the final methyl-arginine products generated. Despite that thousands of substrates have been identified for Type I and II PRMTs, a full scope of arginine methylation catalyzed by the only type III PRMT, PRMT7, as well as its connection with that of type I and II PRMTs remain unknown, limiting our understanding of the network of arginine methylation and its functions in cells. In this study, global profiling of PRMT4 (type I), PRMT5 (type II) and PRMT7 (type III) substrates (also referred as methylome) revealed that PRMT7 methylated a GAR (glycine and arginine) motif similar as PRMT5, while PRMT4 uniquely methylated a motif in which proline was highly enriched. PRMT4, 5 and 7-methylome were all enriched with proteins functioning in mRNA splicing.

2021-01-27 | PXD022424 | Pride

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