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Transcriptome defense response of orange fruit Induced with Rhodoturola mucilaginosa enhanced with salicylic acid

ABSTRACT: Purpose: The goal of this study is to looked at the expression levels of defense genes in orange fruit in response to the application of the antagonist R. mucilaginosa enhanced with SA Methods: Three identical and evenly distributed wounds (5 mm diameter and 3 mm deep) made at the equator of each fruit were inoculated with 30 µL cell suspension of R. mucilaginosa (1×108 cells/mL) + 0.2 mM SA, whiles cell suspension of R. mucilaginosa alone (1×108 cells/mL) was used as a control. Treated fruits were then kept in plastic baskets and covered with film wraps to maintain relative humidity of 95% at storage temperature of 20 ºC. After 72 h of storage the wound tissues were excise and immediately kept in liquid nitrogen and maintained at -80 ºC for the analysis of the RNA. There were three replications per each sample. Results: The results showed that 46 genes were identified as DEGs. Among these 34 were up-regulated and 12 were down regulated, 10 of the RNA-seq these were validated using qRT-PCR. RNA-seq data had a linear relationship withqRT_PCR with a corelation coefficent value of (R2) 0.8388. Conclusion: Our finding showed that the defense genes of orange fruit were up-regulated by the innoculation of R. mucilaginosa enhanced with 0.2 mM SA in the fruit.

ORGANISM(S): Citrus sinensis

PROVIDER: GSE153334 | GEO | 2020/06/27

REPOSITORIES: GEO

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Project description:Background: Anthocyanins are the most important compounds for nutritional quality and economic values of blood orange. However, there are few reports on the pre-harvest treatment accelerate the accumulation of anthocyanins in postharvest blood orange fruit. Here, we performed a comparative Transcriptome and metabolomics analysis to elucidate the underlying mechanism involved in seasonal drought (SD) treatment during fruit expansion stage on anthocyanin accumulation in postharvest ‘Tarocco’ blood orange fruit. Results: Our results showed that SD treatment slowed down the fruit enlargement and increased the sugar accumulation during fruit development and matured period. Obviously, under SD treatment, the accumulation of anthocyanin in blood orange fruit during postharvest storage was significantly accelerated and markedly higher than that in CK. Meanwhile, the total flavonoids and phenols contents and antioxidant activity in SD treatment fruit were also sensibly increased during postharvest storage. Based on metabolome, we found that substrates required for anthocyanin biosynthesis, such as amino acids and their derivatives, and phenolic acids, have significantly accumulated and higher in SD treated mature fruit compared with that of CK. Further according to the results of transcriptome data and weighted gene coexpression correlation network analysis (WGCNA) analysis, phenylalanine ammonia-lyase (PAL3) was considered key structural gene. qRT-PCR analysis verified that the PAL3 was highly expressed in SD treated postharvest stored fruit and was significantly positively correlated with the anthocyanin content. Moreover, we found that other structural genes in anthocyanin biosynthesis pathway were also upregulated under SD treatment through transcriptome data and qRT-PCR analysis. Conclusions: The findings suggest that SD treatment promotes the accumulation of substrates necessary for anthocyanin biosynthesis during fruit ripening process, and activates the expression of anthocyanin biosynthesis pathway genes during postharvest storage period, especially PAL3, co-contributed to the rapid accumulation of anthocyanin. The present study provides a theoretical basis for postharvest quality control and water-saving utilization of blood orange fruit.

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Transcriptome defense response of orange fruit Induced with Rhodoturola mucilaginosa enhanced with salicylic acid

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