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DEtail-seq profiled meiotic DSBs in Saccharomyces cerevisiae

ABSTRACT: SPO11-promoted DNA double-strand breaks (DSBs) formation is a crucial step for meiotic recombination, and it is indispensable to detect the broken DNA ends accurately for dissecting the molecular mechanisms behind. Here, we report a novel technique, named DEtail-seq (DNA End tailing followed by sequencing), that can directly and quantitatively capture the meiotic DSB 3’ overhang hotspots at single-nucleotide resolution.

ORGANISM(S): synthetic construct Escherichia coli Saccharomyces cerevisiae

PROVIDER: GSE154226 | GEO | 2023/01/08

REPOSITORIES: GEO

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Meiotic DNA breaks activate a streamlined response that largely avoids protein level changes -DIA

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