Impact of astrocytic Bmal1 in the circadian profile of transcripts and miRNAs [miRNA-Seq]

ABSTRACT: The circadian clock is an endogenous, self-sustaining oscillator that operates with a periodicity of 24 hours (h) to maintain proper rhythms in gene expression, physiology and behavior. This timekeeping system includes cellular autonomous clocks that are entrained by hormonal and neuronal signals from a central pacemaker located in the suprachiasmatic nuclei (SCN) of the hypothalamus. The molecular mechanisms that drive circadian oscillations in mammalian cells involve self-sustained interlocked transcriptional-translational feedback loops of clock genes/proteins characterized by rhythmic transcription patterns that eventually control downstream rhythmic behavioral patterns and physiological functions within an approximate 24-h period. Nearly half of the circadian output proteome does not show corresponding transcript oscillations, indicating that extensive post-transcriptional mechanisms are important components of circadian rhythmicity. However, the role of brain miRNAs in the generation and maintenance of robust circadian rhythms in animals has not been deeply investigated studied. Core clock genes, such as Bmal1, are expressed in SCN neurons and in other brain cells, such as astrocytes. We previously reported an important role of astrocytes in the control of circadian rhythms at cellular, tissue and organism level (Barca-Mayo et al., Nat. Comm., 2017). Here we used a tamoxifen inducible mouse model (Bmal1flx/flx;GLAST-CreERT2;R26-Tomato, Bmal1cKO), in which we selectivelly delete Bmal1 in approximately 50% of astrocytes, to dissect the impact of astrocyte clocks in the circadian outputs (transcripts and miRNAs) of the cortex.

ORGANISM(S): Mus musculus

PROVIDER: GSE154665 | GEO | 2024/12/31

REPOSITORIES: GEO