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Transcriptome Analysis of Wild Type and Histone H1.2(MeH1.2) Transgenic Plants by RNA-seq in Cassava.

ABSTRACT: Transcriptome Analysis of Wild Type and Histone H1.2(MeH1.2) Transgenic Plants by RNA-seq in Cassava.The goal of this study was to compare the phenotypic and transcriptome differences between transgenic and WT plants, and to analyze how overexpression of MeH1.2 gene changed the expression patterns. MeH1.2 overexpressed transgenic cassava plant (HOE) were was made. The 2-month-old seedlings of HOE were significantly smaller and weaker than of the wild type (WT) under Murashige and Skoog (MS) medium, while, the leave leaf became yellow senescence and the number of branch root increased significantly in HOE than in WT. We found a total of 1670 down-regulated differentially expressed genes (DEGs) and 891 up-regulated DEGs in HOE leave of 2-months-old bottled seedling as indicated by RNA-seq. Many of DEGs were associated with growth metabolism and stress response, such as different amino acids metabolic genes, wound-responsive family genes and peroxidase superfamily genes. We hypothesized the overexpression of MeH1.2 may be similar to mild abiotic stress to retard the growth of HOE, the increased proline content and root-shoot ratio of HOE also support the hypothesis.

ORGANISM(S): Manihot esculenta

PROVIDER: GSE154948 | GEO | 2020/07/24

REPOSITORIES: GEO

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Project description:Title : Characterization of genes differentially expressed in roots of transgenic arabidopsis lines expressing the p25 protein of beet necrotic yellow vein virus. Biological question : Rhizomania ("crazy root") is a severe disease of sugar beet caused by beet necrotic yellow vein virus (BNYVV), which is transmitted by the soil-inhabiting fungus Polymyxa betae. Symptoms of virus infection are characterized by a constricted tap root and a massive proliferation of fine rootlets that often undergo necrosis. BNYVV RNA-3 encodes a 25 kDa (p25) which is an important determinant of leaf symptom phenotype. It also governs BNYVV invasion of the plant root system and induction of rootlet proliferation in sugar beet. In order to obtain a better understanding of molecular aspects of disease development in roots and to characterize specific host genes involved in response to viral infection, transgenic Arabidopsis overexpressors of p25 viral protein was obtained and better characterized. It was shown that transgenic plants that efficiently expressed p25 protein produced more lateral roots. Comparative analysis (microarray) was performed between wild type Arabidopsis roots and transgenic Arabidopsis roots expressing p25 protein, in order to identify Arabidopsis genes differentially expressed in response to p25 viral protein. Experiment description: Seeds were surface sterilized, chilled at 4C for 4 days, and then germinated and grown on square Petri plates containing sterilized Murashige and Skoog (MS) medium with 1% sucrose. Such stock plates were arranged vertically in plastic racks and placed into growth chamber. After 5 days, plants were transferred carefully onto fresh MS medium big round plates. On each plate, 60 Wild Type (WT) plantlets were transferred on the half right of the plate, and 60 transgenic plantlets (B, E or T lines) were transferred on the half left of the plate. Plates were arranged horizontally and placed into growth chamber. Experiment 1 : 5 plates containing WT0A control plants and B0A transgenic plants. Experiment 2 : 5 plates containing WT1 control plants and B transgenic plants. 5 plates containing WT2 control plants and E transgenic plants. 5 plates containing WT3 control plants and T transgenic plants. Plants were harvested after 7 days (experiment 1) or 12 days (experiment 2), and WT roots or transgenic roots were pooled and conserved at -80C.

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Transcriptome Analysis of Wild Type and Histone H1.2(MeH1.2) Transgenic Plants by RNA-seq in Cassava.

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