Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The Single-cell and spatial transcriptional landscape of human development

ABSTRACT: The understanding of human early embryo developoment is very import for us to resovle the mysteries of how human organs especially the brain works and how human disease come up.Therefor, we carried out single cell RNA-seq and spatial transcriptional RNA-seq of human early embryo and analysed gene expression dynamics during the embryo development.We found some import genes and pathways that may control the ongoing of development.These findings help us make a step in understanding how we human being grow from a simple embryo to a complex one.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE155121 | GEO | 2023/05/15

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

The landscape of A-to-I RNA editome in mouse embryonic development

Project description:A-to-I editing, as a post-transcriptional modification process mediated by ADAR, plays a crucial role in many biological process in metazoans.The study of RNA editing in mouse early embryonic development can understand the dynamic changes of RNA editing during mouse early embryonic development, and further reveal the role of RNA editing in mouse early embryo development.We found that as embryonic developing, RNA editome in mice is getting more and more. Besides, RNA editome in mouse embryonic development is stage specified.

2018-11-28 | GSE123012 | GEO

Modeling Post-Implantation Human Development to Yolk Sac Blood Emergence

Project description:Implantation of the human embryo commences a critical developmental stage that comprises profound events including axis formation, gastrulation, and the emergence of yolk sac hematopoietic system. Our mechanistic knowledge of this window of human life remains limited due to restricted access to in vivo samples for both technical and ethical reasons. Stem cell models of human embryo have emerged to help unlock the mysteries of this stage. Here, we present a model of early post-implantation human embryogenesis (embryoid) with an extra-embryonic endoderm and mesoderm niche to support early hematopoiesis. This human embryo model is genetically inducible, and its unanticipated self-organization results in the development of the amniotic cavity, formation of bilaminar disc embryonic morphology, an anterior hypoblast pole and posterior domain. The extra-embryonic layer of iDiscoid presents multilineage yolk sac tissue morphogenesis with extra-embryonic mesoderm and lacks trophoblast. Its development captures distinct waves of yolk sac hematopoiesis including the emergence of erythroid, myeloid and lymphoid cells . iDiscoid offers an easy-to-use, high-throughput, reproducible, and scalable platform to probe multifaceted aspects of human development and blood formation at early post-implantation stage. It will provide a tractable human-based model for drug testing, disease modeling, and a unique cell source for regenerative medicine.

2023-11-10 | GSE247111 | GEO

Time course expression data from early Bovine embryo, Human embryo, and Mouse embryo

Project description:Time course expression data from early Bovine embryo, Human embryo, and Mouse embryo

| PRJNA117925 | ENA

MitoStores: Chaperone-controlled protein granules store mitochondrial precursors in the cytosol - Data Set 1: Global Proteome TMT

Project description:Hundreds of mitochondrial precursor proteins are synthesized in the cytosol and imported into mitochondria in a post-translational reaction. The early stages in mitochondrial protein targeting are poorly understood. Here we show that in baker’s yeast, the cytosol has the capacity to transiently store matrix-destined precursors in dedicated deposits which we named MitoStores. MitoStores are strongly induced when the import into mitochondria is competitively inhibited by clogging the mitochondrial import sites, but also found under physiological conditions when cells grow on non-fermentable carbon sources. MitoStores contain a specific subset of nuclear encoded mitochondrial proteins, in particular those with N-terminal mitochondrial targeting sequences. The cytosolic heat shock proteins Hsp42 and Hsp104 control MitoStore formation, thereby suppressing the toxic potential of accumulating mitochondrial precursor proteins. Thus, the cytosolic protein quality system plays an active role during early stages in mitochondrial protein targeting by the coordinated localized sequestration of mitochondrial precursor proteins.

2023-01-17 | PXD035760 | Pride

MitoStores: Chaperone-controlled protein granules store mitochondrial precursors in the cytosol - Data Set 2: Hsp104 AP-MS

2022-10-15 | PXD035762 | Pride

A developmental model of human early cardiac valvulogenesis (I)

Project description:One third of cardiac congenital diseases affect cardiac valves. Genetically modified mice have advanced our understanding of valve development and related pathologies. However, little is known with regard to human valve development.We aimed to derive a novel human pluripotent stem cell model in order to decode the early steps of human valvulogenesis. Human MesP1+mesodermal cells were differentiated toward the fate of second heart field progenitors and then to a selected population of pre-valvular endocardial cells. Gene arrays revealed that these human prevalvular cells (HPVC) express patterns of genes similar to those expressed in the atrioventricular canal (AVC) endocardium of E9.0 mouse embryos. In mice this developmental time precedes endocardial mesenchymal transition (EndoMT),which is required for mature valve formation. HPVC treated with BMP2, cultured onto chick or mouse AVC cushions, or transplanted into the outflow tract or AVC of embryonic chick and mouse hearts, underwent EndoMTin both a Notch-dependent and independent-manner and expressed markers of valve mesenchymal cells and fibroblasts. Similar to the previously described valve interstitial cells (VICs), HPVC also differentiate into tendinous/chondrogenic cells. Our study indicates tha thuman pluripotent stem cells canrecapitulate early valvulogenesis and thus provide a powerful model to further decipher the origin and lineage contribution of different valvular cell types in humans.

2019-01-22 | GSE73546 | GEO

Mus musculus

Project description:Early mouse embryo development

| PRJNA101341 | ENA

Generation of human blastocyst-like structures from pluripotent stem cells

Project description:Human blastocysts are comprised of the first three cell lineages of the embryo: trophectoderm, epiblast, and primitive endoderm, all of which are essential for early development and organ formation1,2. However, due to ethical concerns and restricted access to human blastocysts, we lack a comprehensive understanding of early human embryogenesis. To bridge this knowledge gap, we need a reliable model system that recapitulates early stages of human embryogenesis. Here we report a ∼three-dimensional (3D), two-step induction protocol for generating blastocyst-like structures (EPS-blastoids) from human extended pluripotent stem (EPS) cells. Morphological and single-cell transcriptomic analyses revealed that EPS-blastoids contain key cell lineages and are transcriptionally similar to human blastocysts. Furthermore, EPS-blastoids also exhibited the developmental potential to undergo post-implantation morphogenesis in vitro to form structures with a cellular composition and transcriptome signature similar to human embryos that had been cultured in vitro for 8 or 10 days. In conclusion, human EPS-blastoids provide a new experimental platform for studying early developmental stages of the human embryo.

2021-05-08 | GSE174034 | GEO

Understanding Early Stage Myelodysplastic Syndrome Pathobiology

Project description:Understanding Early Stage Myelodysplastic Syndrome Pathobiology

| PRJNA476461 | ENA

Adult human brain stem cells 2

Project description:Tissue repair using cell transplantation holds popular appeal. This underlines the need to understand stem cells within the target organ. Our laboratory works on the human brain. Using neurosphere methods, we and others have only been able to passage stem/progenitors a very few times with little expansion of numbers. Now we describe an efficient method for the establishment and propagation of human brain stem cells from whatever tissue samples we have tried. We describe virtually unlimited expansion of an authentic stem cell phenotype. Pluripotency markers Sox2 and Oct4 are expressed without artificial induction. For the first time, multipotency of adult human brain-derived stem cells is demonstrated beyond tissue boundaries. We characterize these cells in detail in vitro including microarray and proteomic approaches. Whilst clarification of these cells' behavior is ongoing, results so far portend well for the future repair of tissues by transplantation of an adult patient's own-derived stem cells. Grey matter and white matter: tissue, stem cells and differentiated cells compared. Total RNA from two brain biopsy sources (grey matter, white matter) from one adult human.

2013-08-19 | E-GEOD-41394 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data