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Exchange of molecular and cellular information: a hybrid model that integrates stem cell divisions and key regulatory interactions

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE155464 | GEO | 2020/12/31

REPOSITORIES: GEO

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Exchange of molecular and cellular information: a hybrid model that integrates stem cell divisions and key regulatory interactions [w5C6]

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Exchange of molecular and cellular information: a hybrid model that integrates stem cell divisions and key regulatory interactions [an3]

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2020-12-31 | GSE155462 | GEO

Exchange of molecular and cellular information: a hybrid model that integrates stem cell divisions and key regulatory interactions

Project description:Exchange of molecular and cellular information: a hybrid model that integrates stem cell divisions and key regulatory interactions

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Exchange of molecular and cellular information: a hybrid model that integrates stem cell divisions and key regulatory interactions [w5C6]

Project description:Exchange of molecular and cellular information: a hybrid model that integrates stem cell divisions and key regulatory interactions [w5C6]

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Exchange of molecular and cellular information: a hybrid model that integrates stem cell divisions and key regulatory interactions [an3]

Project description:Exchange of molecular and cellular information: a hybrid model that integrates stem cell divisions and key regulatory interactions [an3]

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A hybrid model connecting regulatory interactions with stem cell divisions in the root.

Project description:Stem cells give rise to the entirety of cells within an organ. Maintaining stem cell identity and coordinately regulating stem cell divisions is crucial for proper development. In plants, mobile proteins, such as WUSCHEL-RELATED HOMEOBOX 5 (WOX5) and SHORTROOT (SHR), regulate divisions in the root stem cell niche. However, how these proteins coordinately function to establish systemic behaviour is not well understood. We propose a non-cell autonomous role for WOX5 in the cortex endodermis initial (CEI) and identify a regulator, ANGUSTIFOLIA (AN3)/GRF-INTERACTING FACTOR 1, that coordinates CEI divisions. Here, we show with a multi-scale hybrid model integrating ordinary differential equations (ODEs) and agent-based modeling that quiescent center (QC) and CEI divisions have different dynamics. Specifically, by combining continuous models to describe regulatory networks and agent-based rules, we model systemic behaviour, which led us to predict cell-type-specific expression dynamics of SHR, SCARECROW, WOX5, AN3 and CYCLIND6;1, and experimentally validate CEI cell divisions. Conclusively, our results show an interdependency between CEI and QC divisions.

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Project description:Cell division is fundamental to all organisms and the green alga used here exhibits both key animal and plant functions. Specifically, we analyzed the molecular and cellular dynamics of early embryonic divisions of the multicellular green alga Volvox carteri (Chlamydomonadales). Relevant proteins related to mitosis and cytokinesis were identified in silico, the corresponding genes were cloned, fused to yfp, and stably expressed in Volvox, and the tagged proteins were studied by live-cell imaging. We reveal rearrangements of the microtubule cytoskeleton during centrosome separation, spindle formation, establishment of the phycoplast, and generation of previously unknown structures. The centrosomes participate in initiation of spindle formation and determination of spindle orientation. Although the nuclear envelope does not break down during early mitosis, intermixing of cytoplasm and nucleoplasm results in loss of nuclear identity. Finally, we present a model for mitosis in Volvox. Our study reveals enormous dynamics, clarifies spatio-temporal relationships of subcellular structures, and provides insight into the evolution of cell division.

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Patients' support for health information exchange: a literature review and classification of key factors.

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A bacterial control circuit integrates polar localization and proteolysis of key regulatory proteins with a phospho-signaling cascade.

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Molecular and cellular characterization of apoptosis in flat oyster a key mechanisms at the heart of host-parasite interactions.

Project description:Bonamia ostreae has been associated with the decline of flat oyster Ostrea edulis populations in some European countries. This obligatory intracellular parasite persists and multiplies into hemocytes. Previous in vitro experiments showed that apoptosis is activated in hemocytes between 1 h and 4 h of contact with the parasite. The flat oyster uses the apoptosis pathway to defend against B. ostreae. However, the parasite might be also able to modulate this response in order to survive in its host. In order to investigate this hypothesis the apoptotic response of the host was evaluated using flow cytometry, transmission electron microscopy and by measuring the response of genes involved in the apoptotic pathway after 4 h. In parallel, the parasite response was investigated by measuring the expression of B. ostreae genes involved in different biological functions including cell cycle and cell death. Obtained results allow describing molecular apoptotic pathways in O. edulis and confirm that apoptosis is early activated in hemocytes after a contact with B. ostreae. Interestingly, at cellular and molecular levels this process appeared downregulated after 44 h of contact. Concurrently, parasite gene expression appeared reduced suggesting that the parasite could inhibit its own metabolism to escape the immune response.

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